Anti-NGDN 抗体 (ab121124)
Key features and details
- Rabbit polyclonal to NGDN
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NGDN antibody -
製品の詳細
Rabbit polyclonal to NGDN -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 61-209 of Human NGDN (Q8NEJ9).
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ポジティブ・コントロール
- NGDN transfected HEK293T lysate, Human skin tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121124の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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特記事項 |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
ターゲット情報
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機能
Involved in the translational repression of cytoplasmic polyadenylation element (CPE)-containing mRNAs. -
配列類似性
Belongs to the SAS10 family. -
細胞内局在
Nucleus. Cytoplasm. Cell projection > axon. Cell projection > dendrite. Cell projection > filopodium. Detected in axons, dendrites and filopodia. Colocalized with EIF4E in neurites. - Information by UniProt
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参照データベース
- Entrez Gene: 25983 Human
- Omim: 610777 Human
- SwissProt: Q8NEJ9 Human
- Unigene: 9043 Human
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別名
- C14orf120 antibody
- EIF4E-binding protein antibody
- Neuroguidin antibody
see all
画像
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Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus, nucleoli & mitochondria.
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All lanes : Anti-NGDN antibody (ab121124) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : NGDN-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
10-20% SDS PAGE -
ab121124 at 1/350 dilution staining NGDN in Paraffin-emebedded Human skin tissue by Immunohistochemistry. Note: strong nuclear positivity in epidermal cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121124 は論文での使用が確認できていません。