Anti-NFYA 抗体 [EPR9061] (ab139402)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9061] to NFYA
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NFYA antibody [EPR9061]
NFYA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9061] to NFYA -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
適用なし: IHC-P -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human NFYA aa 300-400 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- Recombinant Human NFYA protein (ab131777) can be used as a positive control in WB. NIH 3T3, HeLa, 293T, and Jurkat cell lysates; NIH 3T3 cells. This antibody gave a positive result when used in the following methanol fixed cell lines: Malme-3M
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9061 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab139402の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. -
配列類似性
Belongs to the NFYA/HAP2 subunit family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4800 Human
- Entrez Gene: 18044 Mouse
- Entrez Gene: 29508 Rat
- Omim: 189903 Human
- SwissProt: P23511 Human
- SwissProt: P23708 Mouse
- SwissProt: P18576 Rat
- Unigene: 10441 Human
see all -
別名
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
see all
画像
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All lanes : Anti-NFYA antibody [EPR9061] (ab139402) at 1/1000 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
ab139402 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139402 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Intracellular flow cytometrical analysis of permeabilized NIH 3T3 cells labelling NFYA with ab139402 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab139402 は 2 報の論文で使用されています。
- Nanavaty V et al. DNA Methylation Regulates Alternative Polyadenylation via CTCF and the Cohesin Complex. Mol Cell 78:752-764.e6 (2020). PubMed: 32333838
- Zimmerman D et al. Enhancement of transgene expression by nuclear transcription factor Y and CCCTC-binding factor. Biotechnol Prog 34:1581-1588 (2018). PubMed: 30294957