Anti-NFkB Inducing Kinase NIK 抗体 [3H31L48] (ab203568)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [3H31L48] to NFkB Inducing Kinase NIK
- Suitable for: WB, ICC/IF
- Reacts with: Human
製品の概要
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製品名
Anti-NFkB Inducing Kinase NIK antibody [3H31L48]
NFkB Inducing Kinase NIK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [3H31L48] to NFkB Inducing Kinase NIK -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 700-900.
Database link: Q99558 -
ポジティブ・コントロール
- 293 cells transfected with NIK; FLAG tagged NIK transfected 293 lysate
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
3H31L48 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab203568の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 10 - 20 µg/ml. Predicted molecular weight: 104 kDa.
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ICC/IF |
Use a concentration of 4 - 6 µg/ml.
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特記事項 |
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WB
Use a concentration of 10 - 20 µg/ml. Predicted molecular weight: 104 kDa. |
ICC/IF
Use a concentration of 4 - 6 µg/ml. |
ターゲット情報
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機能
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
組織特異性
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9020 Human
- Omim: 604655 Human
- SwissProt: Q99558 Human
- Unigene: 404183 Human
- Unigene: 668927 Human
- Unigene: 735695 Human
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別名
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
see all
画像
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Lane 1 : anti FLAG tag antibody
Lane 2 : Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (ab203568) at 15 µg/ml
All lanes : Flag tagged NIK transfected 293 cell lysate
Predicted band size: 104 kDa -
Immunohistochemical analysis of non-transfected (left) and NIK transfected 293 cells (right) labeling NIK using ab203568 at 5 μg/mL. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). Hoescht staining of nucleus (blue).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab203568 は 3 報の論文で使用されています。
- Ye F et al. Recruitment of the CoREST transcription repressor complexes by Nerve Growth factor IB-like receptor (Nurr1/NR4A2) mediates silencing of HIV in microglial cells. PLoS Pathog 18:e1010110 (2022). PubMed: 35797416
- Xiao K et al. HuR Affects Proliferation and Apoptosis of Chronic Lymphocytic Leukemia Cells via NF-?B Pathway. Biomed Res Int 2020:1481572 (2020). PubMed: 32908868
- Li XJ et al. Bergapten exerts inhibitory effects on diabetes-related osteoporosis via the regulation of the PI3K/AKT, JNK/MAPK and NF-?B signaling pathways in osteoprotegerin knockout mice. Int J Mol Med N/A:N/A (2016). WB ; Mouse . PubMed: 27840967