製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to NFIL3
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide: RLIATQPISASDSG, corresponding to C terminal amino acids 449-462 of Human NFIL3.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Detects a band of approximately 53 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human NFIL3 peptide (ab22793).

ターゲット情報

  • 機能
    Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.
  • 組織特異性
    Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
  • 配列類似性
    Belongs to the bZIP family. NFIL3 subfamily.
    Contains 1 bZIP domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E4 promoter binding protein 4 antibody
    • E4 promoter-binding protein 4 antibody
    • E4BP 4 antibody
    • E4BP4 antibody
    • E4BP4 protein antibody
    • IL3BP 1 antibody
    • IL3BP1 antibody
    • Interleukin 3 binding protein 1 antibody
    • Interleukin 3 promoter transcriptional activator antibody
    • Interleukin-3 promoter transcriptional activator antibody
    • Interleukin-3-binding protein 1 antibody
    • NFIL 3 antibody
    • NFIL 3A antibody
    • NFIL3 antibody
    • NFIL3_HUMAN antibody
    • NFIL3A antibody
    • Nuclear factor interleukin 3 regulated antibody
    • Nuclear factor interleukin-3-regulated protein antibody
    • Transcriptional activator NF IL3A antibody
    • Transcriptional activator NF-IL3A antibody
    see all

画像

  • Anti-NFIL3 antibody (ab1078) at 0.5 µg/ml + Cell lysate prepared from HepG2 cells at 20 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    The primary antibody incubated for 1 hour.

プロトコール

参考文献

ab1078 has not yet been referenced specifically in any publications.

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. We are very sorry to hear that you are still having difficulty with this antibody. Generally, it is true that PCR results can provide information about the presence of mRNA of a particular target. However, the positive results do not necessary mean that the protein is expressed properly in the cells. Ab1078 has been tested and characterised in Daudi cell lysate, so we would highly suggest using it as positive control. Should you have any special technical enquiry or need any help then please let us know.

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Answer

Dear Dr Salerno I do apologise for my geographical blunder and I hope I haven't caused too much offence. Shipping ab1078 to New Zealand is relatively straight forward. The transit time is about 4 days and shipping charge is GBP45.00. The price again for this item is GBP225.00. You can fax or email your purchase order to us and payment terms are 30 days net. Please let me know if you need further information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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