Application
Western blot
Sample
Human Cell lysate - whole cell (JEG3)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
30 µg
Specification
JEG3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/750
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP: sc-2004
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
blocking with immunizing peptide ab4978 to detect specific band
Exposure
6 minute(s) and 40 second(s)
Bands
Specific: 230 kDa Non-specific: 180, 120, 70, 50, 30 kDa
Positive control
rat kidney
Additional data
Additional Notes
1+2) JEG3 whole cell lysate;
3) rat placenta lysate;
4) rat kidney lysate;
left: ab3446 + immunizing peptide ab4978;
right: ab3446;
MW markers from top to bottom: 250, 130, 100, 55, 35 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 20 2017