Anti-NFAT2 抗体 (ab25916)
Key features and details
- Rabbit polyclonal to NFAT2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human, Chimpanzee
- Isotype: IgG
製品の概要
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製品名
Anti-NFAT2 antibody
NFAT2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NFAT2 -
由来種
Rabbit -
特異性
The amino acid sequence used is 100% identical in human NFATc1A, B, C and E isoforms. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon the cell lines. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human, Chimpanzee -
免疫原
Synthetic peptide:
NKRKYSLNGRQPPYSPHHS
, corresponding to amino acids 263-282 of Human NFATc1 -
ポジティブ・コントロール
- Ramos cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25916の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
1/20.
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WB | (4) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight varies with isoform.
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特記事項 |
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ICC/IF
1/20. |
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight varies with isoform. |
ターゲット情報
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機能
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. -
組織特異性
Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. -
配列類似性
Contains 1 RHD (Rel-like) domain. -
ドメイン
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor. -
翻訳後修飾
Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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参照データベース
- Entrez Gene: 4772 Human
- Entrez Gene: 18018 Mouse
- Omim: 600489 Human
- SwissProt: O95644 Human
- SwissProt: O88942 Mouse
- Unigene: 534074 Human
- Unigene: 701518 Human
- Unigene: 329560 Mouse
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別名
- cytoplasmic 1 antibody
- MGC138448 antibody
- NF ATc antibody
see all
画像
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All lanes : Anti-NFAT2 antibody (ab25916) at 1 mg/ml
Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate -
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for NFAT2 (green) using ab25916 at 1/500 dilution in ICC/IF.
The nuclear counterstain is DAPI (blue). Alpha-Tubulin is stained with Dylight 550 (red).
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Western blot analysis of NFATc1 in Ramos cell lysate. Lane 1. Without blocking peptide. Lane 2. With blocking peptide.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (52)
ab25916 は 52 報の論文で使用されています。
- Zhang W et al. Reversing the imbalance in bone homeostasis via sustained release of SIRT-1 agonist to promote bone healing under osteoporotic condition. Bioact Mater 19:429-443 (2023). PubMed: 35574058
- Ding C et al. Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection. FASEB J 36:e22468 (2022). PubMed: 35913801
- Chen X et al. Macrophage M1 polarization mediated via the IL-6/STAT3 pathway contributes to apical periodontitis induced by Porphyromonas gingivalis. J Appl Oral Sci 30:e20220316 (2022). PubMed: 36417596
- Tian Y et al. Immunosuppressants Tacrolimus and Sirolimus revert the cardiac antifibrotic properties of p38-MAPK inhibition in 3D-multicellular human iPSC-heart organoids. Front Cell Dev Biol 10:1001453 (2022). PubMed: 36438566
- Xie ZY et al. NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury. Acta Pharmacol Sin 43:2081-2093 (2022). PubMed: 34937917