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  1. Link

    nfat2-antibody-7a6-ab2796.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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Anti-NFAT2 抗体 [7A6] (ab2796)

  • Datasheet
  • SDS
Reviews (9)Q&A (8)References (29)

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出荷および受注について

Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
  • Flow Cytometry - Anti-NFAT2 antibody [7A6] (ab2796)

Key features and details

  • Mouse monoclonal [7A6] to NFAT2
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Hamster, Human, Non human primates
  • Isotype: IgG1

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
タンパク質
Product image
Recombinant Human NFAT2 protein (ab64307)

関連製品

製品の概要

  • 製品名

    Anti-NFAT2 antibody [7A6]
    NFAT2 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [7A6] to NFAT2
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Hamster, Human, Non human primates
  • 免疫原

    Fusion protein corresponding to NFAT2 aa 1-700.

  • ポジティブ・コントロール

    • IHC-P: FFPE human Hodgkin's lymphoma tissue sections.
  • 特記事項

    This monoclonal antibody is manufactured by Abcam. If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find more information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    7A6
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFATS
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFATs
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

関連製品

  • Alternative Versions

    • Anti-NFAT2 antibody [7A6] - BSA and Azide free (ab264530)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human NFAT2 protein (ab64307)

アプリケーション

Our Abpromise guarantee covers the use of ab2796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
  • 組織特異性

    Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
  • 配列類似性

    Contains 1 RHD (Rel-like) domain.
  • ドメイン

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
    Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
  • 翻訳後修飾

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • 細胞内局在

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Target information above from: UniProt accession O95644 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 4772 Human
    • Entrez Gene: 18018 Mouse
    • Entrez Gene: 100361818 Rat
    • Omim: 600489 Human
    • SwissProt: O95644 Human
    • SwissProt: O88942 Mouse
    • Unigene: 534074 Human
    • Unigene: 701518 Human
    • Unigene: 329560 Mouse
    • Unigene: 229098 Rat
    see all
  • 別名

    • cytoplasmic 1 antibody
    • MGC138448 antibody
    • NF ATc antibody
    • NF ATc1 antibody
    • NF-ATc antibody
    • NF-ATc1 antibody
    • NF-ATc1.2 antibody
    • NFAC1_HUMAN antibody
    • NFAT 2 antibody
    • NFAT transcription complex cytosolic component antibody
    • NFATC 1 antibody
    • NFATc antibody
    • NFATc1 antibody
    • Nuclear factor of activated T cells cytoplasmic 1 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
    • Nuclear factor of activated T cells cytosolic component 1 antibody
    • nuclear factor of activated T-cells 'c' antibody
    • Nuclear factor of activated T-cells antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)

    IHC image of NFAT2 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2796, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)

    ab2796 staining human normal tonsil tissue. Staining is localized to cytoplasm and nucleus.
    Left panel: with primary antibody at 1 µg/ml. Right panel: Isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX.

    Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Flow Cytometry - Anti-NFAT2 antibody [7A6] (ab2796)
    Flow Cytometry - Anti-NFAT2 antibody [7A6] (ab2796)

    Overlay histogram showing Jurkat cells stained with ab2796 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2796, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

プロトコール

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (29)

    ab2796 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2796 は 29 報の論文で使用されています。

    • Mira-Pascual L  et al. A Sub-Clone of RAW264.7-Cells Form Osteoclast-Like Cells Capable of Bone Resorption Faster than Parental RAW264.7 through Increased De Novo Expression and Nuclear Translocation of NFATc1. Int J Mol Sci 21:N/A (2020). PubMed: 31947698
    • Meng J  et al. Stachydrine prevents LPS-induced bone loss by inhibiting osteoclastogenesis via NF-?B and Akt signalling. J Cell Mol Med 23:6730-6743 (2019). PubMed: 31328430
    • Soares MPR  et al. The use of apocynin inhibits osteoclastogenesis. Cell Biol Int N/A:N/A (2019). PubMed: 30761659
    • Oh-Hora M  et al. Stromal Interaction Molecule Deficiency in T Cells Promotes Spontaneous Follicular Helper T Cell Development and Causes Type 2 Immune Disorders. J Immunol 202:2616-2627 (2019). PubMed: 30910863
    • Sharlo K  et al. Plantar mechanical stimulation prevents calcineurin-NFATc1 inactivation and slow-to-fast fiber type shift in rat soleus muscle under hindlimb unloading. J Appl Physiol (1985) 126:1769-1781 (2019). PubMed: 31046517
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 17 Abreviews or Q&A

    Western blot abreview for Anti-NFAT2 antibody [7A6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 17 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-NFAT2 antibody [7A6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (palatine tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    palatine tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 26 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Hannes Kaddatz

    Verified customer

    投稿 Jan 02 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Liver)
    Permeabilization
    Yes - 0.1%Triton x-100 in PB
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 16 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Umair Latif

    Verified customer

    投稿 Jun 18 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hu pluripotent stem cell derived epicardial like)
    Permeabilization
    Yes - saponin
    Specification
    Hu pluripotent stem cell derived epicardial like
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 06 2016

    Question

    Does this antibody bind to the phosphorylated (cytoplasmic) or unphosphorylated (nuclear) form of NFAT2?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2013

    Answer

    Anti-NFAT2 [7A6] antibody - ChIP Grade, ab2796 binds to the cytoplasmic form within amino acids 1-654.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Dec 05 2013

    Question

    Please can you let me know what the antibody concentration is for: Anti-NFAT2 antibody [7A6] - ChIP Grade (ab2796).
    Many Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 03 2012

    Answer

    Thank you for your enquiry.

    I can confirm that ab2796 NFAT2 antibody [7A6] antibody is sold asascites fluid. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for ascites, concentration of antibody is known to very between 5 - 10 mg/ml.

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Sep 03 2012

    Question

    Inquiry: Kindly advise currently available lot of ab2796. Thanks in advance for your assistance and reply.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2012

    Answer

    Thank you for your enquiry.

    The lot we currently have available is #1312558 (GR73411-4).

    I hope this information helps.

    Read More

    Abcam Scientific Support

    Answered on Jul 02 2012

    1-10 of 17 Abreviews or Q&A

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