Application
Western blot
Sample
Mouse Tissue lysate - whole (nerves)
Loading amount
40 µg
Specification
nerves
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECLplus
Exposure
40 second(s)
Bands
Specific: 80 kDa Non-specific: 100 kDa
Additional data
Additional Notes
Lane1: normal tissue
Lane2: the tissue in which Pak activity was low
Lane2: the tissue in which Pak activity was low
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 09 2009