Application
ChIP
Sample
Human Cell lysate - nuclear (Kidney (Clear Cell Renal Carcinoma))
Negative control
786-O clear cell renal carcinoma cells.
Specification
Kidney (Clear Cell Renal Carcinoma)
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Metastatic renal cancer cell obtained from 786-O parental line.
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
Validated with ChIP-seq, 50ug antibody per IP in a total of 70 million cells.
PubMed ID
29875134: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 17 2018