Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Dilution
1/500
Incubation time
30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
I also tested this antibody in a Western blot of whole cell extracts from HeLa and HCT116 cells. It gives a very specific signal at ~65kDa and also appears to give a faint background band at ~30kDa.
I also immunoflourescently labelled Indian muntjac (IM) cells with ab16502 where it gives a predominantly cytoplasmic focal pattern. The image is an interphase IM cell labelled with anti-NFKB p65 at 1:500.
I also immunoflourescently labelled Indian muntjac (IM) cells with ab16502 where it gives a predominantly cytoplasmic focal pattern. The image is an interphase IM cell labelled with anti-NFKB p65 at 1:500.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Feb 27 2006