Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Pattern of immunopositivity appears to be very similar to that of anti Myeloperoxidase ( ab45977 - abreview image is of a near-sequential section from same Pwax block)
Abcam response
Many thanks for your positive feedback on ab68672 (Neutrophil Elastase antibody) in IHC-P on human spleen tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Apr 21 2010