Anti-Neutrophil 抗体 [NIMP-R14] (ab2557)
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
製品の概要
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製品名
Anti-Neutrophil antibody [NIMP-R14]
Neutrophil 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [NIMP-R14] to Neutrophil -
由来種
Rat -
アプリケーション
適用あり: IHC-Frmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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エピトープ
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
特記事項
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
ポリ/モノ
モノクローナル -
クローン名
NIMP-R14 -
ミエローマ
210RCY3-Ag123 -
アイソタイプ
IgG2b -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr | Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
ターゲット情報
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細胞内局在
Cell membrane; Lipid-anchor, GPI-anchor -
参照データベース
- Entrez Gene: 110453 Mouse
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別名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
画像
プロトコール
参考文献 (166)
ab2557 は 166 報の論文で使用されています。
- Qian J et al. Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway. Biomed Res Int 2020:3635787 (2020). PubMed: 32258116
- Kim KW et al. FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress. Cell Death Dis 11:285 (2020). PubMed: 32332708
- Ryu G et al. Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp. Allergy Asthma Immunol Res 12:507-522 (2020). PubMed: 32141263
- Park CJ et al. Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation. Cell Rep 31:107496 (2020). PubMed: 32294429
- Kong AM et al. Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle. Acta Biomater 94:281-294 (2019). PubMed: 31152943