Anti-Neutrophil 抗体 [NIMP-R14] (ab2557)
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: Functional Studies, IHC-P, Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
製品の概要
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製品名
Anti-Neutrophil antibody [NIMP-R14]
Neutrophil 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [NIMP-R14] to Neutrophil -
由来種
Rat -
アプリケーション
適用あり: Functional Studies, IHC-P, Flow Cyt, IHC-Frmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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エピトープ
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
特記事項
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
ポリ/モノ
モノクローナル -
クローン名
NIMP-R14 -
ミエローマ
210RCY3-Ag123 -
アイソタイプ
IgG2b -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. | |
| IHC-P | Use at an assay dependent concentration. This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
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| Flow Cyt | Use at an assay dependent concentration. 5 x 105 cells were incubated with 10 µg/ml antibody. |
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| IHC-Fr | Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
ターゲット情報
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細胞内局在
Cell membrane; Lipid-anchor, GPI-anchor -
参照データベース
- Entrez Gene: 110453 Mouse
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別名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-320034-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Mukai K et al. The effect of 17ß-estradiol on cutaneous wound healing in protein-malnourished ovariectomized female mouse model. PLoS One 9:e115564 (2014). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/4% paraformaldehyde-fixed mouse uterus tissue 3 or 7 days after wounding, stained for Neutrophil using ab2557 at 1/100 dilution in immunohistochemical analysis.
Slides from let to right include: Control SHAM, Protein-malnurished SHAM, protein-malnourished ovariectomized (OVX) female mice, and PM OVX mice treated with 17β-estradiol.
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![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-260762-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)This image is courtesy of an anonymous Abreviewab2557 staining Neutrophil in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM sodium citrate. Samples were incubated with primary antibody (1/200 in 1x PBS/1% BSA/0.3% Triton X-100 buffer) for 1 hour at 25°C. A HRP-conjugated Goat anti-rat Ig polyclonal (1/200) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-5943-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Image courtesy of an anonymous Abreview.ab2557 staining Neutrophil in mouse tumor tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked, followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes. A biotin-conjugated rabbit anti-rat was used as secondary antibody at a 1/400 dilution.
プロトコール
参考文献
ab2557 は 157 報の論文で使用されています。
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- Hong JM et al. SB365, Pulsatilla Saponin D Induces Caspase-Independent Cell Death and Augments the Anticancer Effect of Temozolomide in Glioblastoma Multiforme Cells. Molecules 24:N/A (2019). PubMed: 31491945
