Anti-Neutrophil 抗体 [NIMP-R14] (ab2557)
![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)")
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: ICC, IHC-Fr, IHC-P, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Neutrophil antibody [NIMP-R14]
Neutrophil 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [NIMP-R14] to Neutrophil -
由来種
Rat -
アプリケーション
適用あり: ICC, IHC-Fr, IHC-P, Flow Cyt, Functional Studiesmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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エピトープ
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
特記事項
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
ポリ/モノ
モノクローナル -
クローン名
NIMP-R14 -
ミエローマ
210RCY3-Ag123 -
アイソタイプ
IgG2b -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | (3) |
Use at an assay dependent concentration.
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| IHC-Fr | (4) |
Use at an assay dependent concentration. Tested with acetone-fixed tissue.
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| IHC-P | (15) |
Use at an assay dependent concentration.
This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
| Flow Cyt | (1) |
Use at an assay dependent concentration.
5 x 10^5 cells were incubated with 10 ug/ml antibody. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Functional Studies |
Use at an assay dependent concentration.
Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. |
| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
|
IHC-P
Use at an assay dependent concentration. This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
|
Flow Cyt
Use at an assay dependent concentration. 5 x 10^5 cells were incubated with 10 ug/ml antibody. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
Functional Studies
Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. |
ターゲット情報
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細胞内局在
Cell membrane; Lipid-anchor, GPI-anchor -
参照データベース
- Entrez Gene: 110453 Mouse
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別名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
画像
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Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (200)
ab2557 は 200 報の論文で使用されています。
- Eliseeva SI et al. Changes in lung immune cell infiltrates after electric field treatment in mice. Sci Rep 11:1453 (2021). PubMed: 33446928
- Molenaar B et al. Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair. Commun Biol 4:146 (2021). PubMed: 33514846
- Miao AF et al. Hypoxia-inducible factor prolyl hydroxylase inhibitor roxadustat (FG-4592) protects against renal ischemia/reperfusion injury by inhibiting inflammation. Ren Fail 43:803-810 (2021). PubMed: 33966598
- Brunner SM et al. Galanin receptor 3 attenuates inflammation and influences the gut microbiota in an experimental murine colitis model. Sci Rep 11:564 (2021). PubMed: 33436730
- Hazlewood JE et al. Injection site vaccinology of a recombinant vaccinia-based vector reveals diverse innate immune signatures. PLoS Pathog 17:e1009215 (2021). PubMed: 33439897