製品の概要
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製品名Anti-Neutrophil antibody [NIMP-R14]
Neutrophil 一次抗体 製品一覧 -
製品の詳細Rat monoclonal [NIMP-R14] to Neutrophil
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由来種Rat
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アプリケーション適用あり: ICC, Functional Studies, IHC-P, Flow Cyt, IHC-Frmore details
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種交差性交差種: Mouse
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免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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エピトープThe monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
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特記事項
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度Protein G purified
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一次抗体 備考The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
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ポリ/モノモノクローナル
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クローン名NIMP-R14
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ミエローマ210RCY3-Ag123
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アイソタイプIgG2b
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軽鎖の種類unknown
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab2557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection. | |
| IHC-P | Use at an assay dependent concentration. This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required. |
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| Flow Cyt | Use at an assay dependent concentration. 5 x 105 cells were incubated with 10 µg/ml antibody. |
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| IHC-Fr | Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
ターゲット情報
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細胞内局在Cell membrane; Lipid-anchor, GPI-anchor
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参照データベース
- Entrez Gene: 110453 Mouse
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別名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
画像
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![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-282294-2557.jpg)
Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
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![Immunocytochemistry - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557_1.jpg)
Immunocytochemistry - Anti-Neutrophil antibody [NIMP-R14] (ab2557)ab2557 at 1/80 staining mouse blood cells (ip wash cells) by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a biotinylated rabbit anti-rat (mouse adsorbed) antibody.
This image is courtesy of an Abreview submitted on 16 March 2006.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-260762-ab2557IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)This image is courtesy of an anonymous Abreviewab2557 staining Neutrophil in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM sodium citrate. Samples were incubated with primary antibody (1/200 in 1x PBS/1% BSA/0.3% Triton X-100 buffer) for 1 hour at 25°C. A HRP-conjugated Goat anti-rat Ig polyclonal (1/200) was used as the secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/Neutrophil-Primary-antibodies-ab2557-5.jpg)
Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)ab2557 staining Ly-6G/-6C in mouse spleen tissue by Immunohistochemistry (Frozen sections). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/Neutrophil-Primary-antibodies-ab2557-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)Image courtesy of an anonymous Abreview.ab2557 staining Neutrophil in mouse tumor tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked, followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes. A biotin-conjugated rabbit anti-rat was used as secondary antibody at a 1/400 dilution.
プロトコール
参考文献
This product has been referenced in:
- Bodas M et al. Inhibition of histone-deacetylase activity rescues inflammatory cystic fibrosis lung disease by modulating innate and adaptive immune responses. Respir Res 19:2 (2018). IHC-P ; Mouse . Read more (PubMed: 29301535) »
- Mahmutovic Persson I et al. IL-1ß mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation. Respir Res 19:16 (2018). IHC-P ; Mouse . Read more (PubMed: 29361942) »
レビューと Q&A
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