神経上皮細胞マーカー


神経上皮細胞は未分化な細胞で、ニューロンやグリア細胞などの中枢神経系、脊髄、網膜などはこの細胞に由来します。この細胞は外胚葉由来で、胎生初期には脳室帯に局在します。またお互いに密着結合する性質があり、頂底軸極性(apical-basal polarity)を持ち、細胞周期の進行に伴う核移動(INM)を示します。

核移動とは、細胞周期の S 期で細胞の頂端に局在する核が M 期に基底側に移動することです(G1 期および G2 期においてはその中間領域に存在します)。このような運動は、Notch シグナルなどの増殖シグナルを核に伝達しやすくするために起こっていると考えられています。

ここでは、この Notch を代表とする神経上皮細胞の主なマーカー・タンパク質と、それらに対する抗体をご紹介します。


Nestin
An intermediate filament protein expressed in NE cells. Its expression persists in radial glia until astrocyte development.

iPSCs stained with anti-nestin (red)(ab105389).

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SOX2
A transcription factor and the earliest marker of the neural plate. It is expressed in proliferating cells and those that acquire glial fates, but downregulated in post-mitotic neurons.

E13 mouse spinal cord sections stained with anti-SOX2 (ab97959).

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Notch1
A transmembrane receptor that regulates the formation, migration, and differentiation of neural crest cells.

Mouse embryo tissue sections stained with anti-notch1 (green) (ab65297).

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Notch signaling inhibitor: FLI-06 (ab146170)


HES1 and HES3
Transcription factors that maintain the symmetrical cell division of NE cells. When NE cells become radial glia, HES3 is downregulated and HES5 is upregulated.

Mouse NIH3T3 cells stained with anti-HES1 (green) (ab71559).

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Occludin
A tight junction protein lost after neural tube closure prior to neurogenesis.

Human breast cancer cells stained with anti-occludin (red) (ab31721).

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E-cadherin
Cell adhesion molecule downregulated after neural tube closure prior to neurogenesis.

Human pluripotent stem cells stained with anti-E-cadherin (ab40772).

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SOX10
A transcription factor present in migrating neural crest cells.

A375 cells stained with anti-SOX10 (ab155279).

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参考文献

  • Bylund, M., Andersson, E., Novitch, B. G. & Muhr, J. Vertebrate neurogenesis is counteracted by Sox1-3 activity. Nat Neurosci 6, 1162–1168 (2003).
  • Jessen, K. R. & Mirsky, R. The origin and development of glial cells in peripheral nerves. Nat. Rev. Neurosci. 6, 671–82 (2005).
  • Krämer, A. et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat. Chem. Biol. 9, 731–8 (2013).
  • Kageyama, R., Ohtsuka, T. & Kobayashi, T. Roles of Hes genes in neural development. Development Growth and Differentiation 50, (2008).
  • Kriegstein, A. R. & Götz, M. Radial glia diversity: A matter of cell fate. Glia 43, 37–43 (2003).
  • Noisa, P., Lund, C., Kanduri, K., Lund, R. & La, H. Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells. J. Cell Sci. 127, 2083–2094 (2014).
  • Papanayotou, C. et al. A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. Plos Biol 6, e2 (2008).


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