未熟ニューロンと Intermediate progenitor マーカー


放射状グリアの非対称分裂により産生された intermediate progenitor 細胞(IPC)はその後、未熟ニューロン(immature neuron)へと分化します。未熟ニューロンは、文字通り未成熟なニューロンですが、同じく未成熟な神経系の細胞である神経幹細胞や放射状グリア細胞とは異なり、これ以上細胞分裂は起こしません。神経系を移動して目的地に達した後、神経突起を伸展させてシナプス結合し、最終的に神経回路網の一員となります。

形態的には成熟ニューロンと比較して樹状突起がシンプルで、また NeuN や MAP-2 などといった、成熟ニューロンで見られるようなマーカー・タンパク質のいくつかは発現していません。

ここでは未熟ニューロンと intermediate progenitor 細胞の主なマーカー・タンパク質と、それらに対する抗体を紹介します。


Intermediate progenitors

TBR2
A transcription factor whose expression marks the transition from radial glia to intermediate progenitors.

Embryonic (E15) mouse cortical sections stained with anti-TBR2 (red) (ab23345).

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MASH1 (Ascl1)
A transcription factor essential for neural differentiation. Please note that it can also label active neural stem cells.

Mouse subventricular zone tissue sections stained with anti-MASH1 (ab74065).

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Immature neurons

Doublecortin
A microtubule-associated phosphoprotein that promotes neurite extension and cell migration.

Mouse adult dentate gyrus sections stained with anti-doublecortin (red) (ab18723).

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Beta III tubulin
A class of tubulin whose expression is limited to neurons and is used to label immature neurons.

Mouse embryonic spinal cord stained with anti-beta III tubulin (red) (ab78078).

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NeuroD1
A transcription factor that promotes neuronal development.

Mouse brain tissue stained with anti-neuroD1 (ab60704).

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TBR1
A transcription factor whose expression marks the transition from intermediate progenitors to post-mitotic neurons.

Mouse brain sections stained with anti-TBR1 (red) (ab31940).

Browse all TBR1 antibodies


Stathmin 1
A cytoplasmic phosphoprotein involved in cytoskeletal regulation.

Mouse brain tissue sections stained with anti-stathmin 1 (ab109986).

Browse all stathmin 1 antibodies


参考文献

  • Atweh, C. The Role of Stathmin in the Regulation of the Cell Cycle. J.Cell.Biochem 93, (2004).
  • Englund, C. et al. Pax6, Tbr2, and Tbr1 Are Expressed Sequentially by Radial Glia, Intermediate Progenitor Cells, and post-mitotic Neurons in Developing Neocortex. J. Neurosci. 25, 247–251 (2005).
  • Katsetos, C. D. et al. Class III beta-tubulin isotype (beta III) in the adrenal medulla: I. Localization in the developing human adrenal medulla. Anat. Rec. 250, 335–43 (1998).
  • Kim, E. J., Ables, J. L., Dickel, L. K., Eisch, A. J. & Johnson, J. E. Ascl1 (Mash1) Defines Cells with Long-Term Neurogenic Potential in Subgranular and Subventricular Zones in Adult Mouse Brain. (2011).
  • Kwak, M. et al. Effects of Neonatal Hypoxic-Ischemic Injury and Hypothermic Neuroprotection on Neural Progenitor Cells in the Mouse Hippocampus. Dev. Neurosci. 37, 428–439 (2015).
  • Martí-Mengual, U., Varea, E., Crespo, C., Blasco-Ibáñez, J. M. & Nacher, J. Cells expressing markers of immature neurons in the amygdala of adult humans. Eur. J. Neurosci. 37, 10–22 (2013).
  • Menezes, J. R. & Luskin, M. B. Expression of neuron-specific tubulin defines a novel population in the proliferative layers of the developing telencephalon. J. Neurosci. 14, 5399–5416 (1994).
  • Pollen, A. A. et al. Molecular Identity of Human Outer Radial Glia during Cortical Development. Cell 163, 55–67 (2015).
  • Shin, J. et al. Single-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis. Cell Stem Cell 17, 360–372 (2015).
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