Anti-Neuropeptide S 抗体 (ab18252)
Key features and details
- Rabbit polyclonal to Neuropeptide S
- Suitable for: IHC (PFA fixed), RIA
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Neuropeptide S antibody
Neuropeptide S 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neuropeptide S -
由来種
Rabbit -
特異性
This antibody is generated against the complete 20aa sequence of the fully processed rat NPS peptide, it should run at about 2kDa on WB. ab18252 detects mouse NPS peptide in IHC (PFA fixed) and in RIA in hypothalamic mouse brain tissue. -
アプリケーション
適用あり: IHC (PFA fixed), RIAmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC (PFA fixed) |
Use a concentration of 0.3 µg/ml.
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| RIA |
Use a concentration of 0.1 - 0.3 µg/ml.
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| 特記事項 |
|---|
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IHC (PFA fixed)
Use a concentration of 0.3 µg/ml. |
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RIA
Use a concentration of 0.1 - 0.3 µg/ml. |
ターゲット情報
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機能
Modulates arousal and anxiety. May play an important anorexigenic role (By similarity). Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations (PubMed:15312648, PubMed:16790440). -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 100043254 Mouse
- Entrez Gene: 100360071 Rat
- SwissProt: P0C0P8 Mouse
- SwissProt: P0C0P7 Rat
- Unigene: 192092 Mouse
- Unigene: 234299 Rat
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別名
- Neuropeptide S antibody
- Nps antibody
- NPS_HUMAN antibody
画像
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Immunohistochemistry (PFA fixed) - Anti-Neuropeptide S antibody (ab18252)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom.
Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
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Radioimmunoassay - Anti-Neuropeptide S antibody (ab18252)IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham).
Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).
データシートおよび資料
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Datasheet download
参考文献 (7)
ab18252 は 7 報の論文で使用されています。
- Yang WZ et al. Parabrachial neuron types categorically encode thermoregulation variables during heat defense. Sci Adv 6:N/A (2020). PubMed: 32917598
- Breton-Provencher V & Sur M Active control of arousal by a locus coeruleus GABAergic circuit. Nat Neurosci 22:218-228 (2019). IHC-FoFr ; Mouse . PubMed: 30643295
- Tillmann S et al. Sustained overexpression of neuropeptide S in the amygdala reduces anxiety-like behavior in rats. Behav Brain Res 367:28-34 (2019). PubMed: 30914309
- Grund T et al. Neuropeptide S Activates Paraventricular Oxytocin Neurons to Induce Anxiolysis. J Neurosci 37:12214-12225 (2017). Rat . PubMed: 29118105
- Adori C et al. Neuropeptide S- and Neuropeptide S receptor-expressing neuron populations in the human pons. Front Neuroanat 9:126 (2015). IHC ; Rat . PubMed: 26441556
- Liu X et al. Molecular fingerprint of neuropeptide S-producing neurons in the mouse brain. J Comp Neurol 519:1847-66 (2011). Mouse . PubMed: 21452230
- Clark SD et al. Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry. J Comp Neurol 519:1867-93 (2011). Mouse . PubMed: 21452235
