Anti-Neuron specific beta III Tubulin 抗体 (ab229590)
Key features and details
- Rabbit polyclonal to Neuron specific beta III Tubulin
- Suitable for: ICC/IF, IP, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neuron specific beta III Tubulin antibody -
製品の詳細
Rabbit polyclonal to Neuron specific beta III Tubulin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, IHC-P, WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Neuron specific beta III Tubulin (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q13509 -
ポジティブ・コントロール
- WB: HeLa, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; Mouse and rat brain tissue extracts. IP: SK-N-SH whole cell extract. IHC-P: Rat brain tissue. IHC-Fr: E13.5 rat brain tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab229590の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/50000.
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IHC-Fr |
1/100 - 1/1000.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/50000. |
IHC-Fr
1/100 - 1/1000. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
組織特異性
Expression is primarily restricted to central and peripheral nervous system. -
関連疾患
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Entrez Gene: 246118 Rat
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- SwissProt: Q4QRB4 Rat
- Unigene: 511743 Human
see all -
別名
- beta 4 antibody
- MC1R antibody
- TBB3_HUMAN antibody
see all
画像
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All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Neuron specific beta III Tubulin Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG -
All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Neuron specific beta III Tubulin knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.10% SDS-PAGE gel.
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Frozen E13.5 rat brain tissue stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue).
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4 % paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Paraffin-embedded rat brain tissue stained for Neuron specific beta III Tubulin using ab229590 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.10% SDS-PAGE gel.
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All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/50000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.10% SDS-PAGE gel.
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Neuron specific beta III Tubulin was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229590. Western blot was performed from the immunoprecipitate using ab229590. Anti-rabbit IgG was used as a secondary antibody.
Lane 1: SK-N-SH whole cell extract (Input).
Lane 2: Control IgG instead of ab229590 in SK-N-SH whole cell extract.
Lane 3: ab229590 IP in SK-N-SH whole cell extract.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab229590 は 3 報の論文で使用されています。
- Poon A et al. The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons. Stem Cell Reports 16:1276-1289 (2021). PubMed: 33891871
- Stewart AN et al. Transplantation of Mesenchymal Stem Cells that Overexpress NT-3 Produce Motor Improvements without Axonal Regeneration following Complete Spinal Cord Transections in Rats. Brain Res N/A:N/A (2018). IHC-P . PubMed: 29883625
- Bao N et al. Upregulation of miR-199a-5p Protects Spinal Cord Against Ischemia/Reperfusion-Induced Injury via Downregulation of ECE1 in Rat. Cell Mol Neurobiol 38:1293-1303 (2018). PubMed: 29948551