Anti-NK-2R 抗体 (ab233295)
Key features and details
- Rabbit polyclonal to NK-2R
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-NK-2R antibody
NK-2R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NK-2R -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human, Pig -
免疫原
Recombinant fragment (His-tag) corresponding to Human NK-2R aa 50-300. Expressed in E.coli. N-terminal tag.
Database link: P21452 -
ポジティブ・コントロール
- WB: Human lung lysate; HeLa cell lysate; Pig small intestine and heart lysate; Recombinant human NK-2R protein. IHC-P: Human pancreatic cancer, breast cancer and stomach tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
ターゲット情報
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機能
This is a receptor for the tachykinin neuropeptide substance K (neurokinin A). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance K > neuromedin-K > substance P. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6865 Human
- Omim: 162321 Human
- SwissProt: P21452 Human
- Unigene: 88372 Human
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別名
- Neurokinin 2 receptor antibody
- Neurokinin A receptor antibody
- NK 2 receptor antibody
see all
画像
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Formalin-fixed, paraffin-embedded human stomach tissue stained for NK-2R using ab233295 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NK-2R using ab233295 (green) at 10 µg/mL in ICC/IF. FITC-labeled secondary antibody.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for NK-2R using ab233295 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for NK-2R using ab233295 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-NK-2R antibody (ab233295) at 1 µg/ml + Pig heart lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 1 µg/ml + Pig small intestine lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 1 µg/ml + Human lung lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 2 µg/ml + Recombinant human NK-2R protein
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab233295 は論文での使用が確認できていません。