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リコンビナントRabMAb

Anti-Neurogenin 2/NGN2 抗体 [EPR4177] (ab109236)

Reviews (1)Q&A (1)References (1)
Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
  • Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Neurogenin 2/NGN2 antibody [EPR4177]
    Neurogenin 2/NGN2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: SH-SY5Y and HepG2 cell lysates. ICC/IF: SH-SY-5Y cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109236の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

画像

  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified)

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/1000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa

  • Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
    Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)

参考文献 (1)

ab109236 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109236 は 1 報の論文で使用されています。

  • Di Carlo V  et al. TDP-43 regulates the microprocessor complex activity during in vitro neuronal differentiation. Mol Neurobiol 48:952-63 (2013). PubMed: 24113842

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Neurogenin 2/NGN2 antibody [EPR4177]

 Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - 0.3% triton
Specification
Retina
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 21 2014

Question

Customer kindly contacted us that ab109236 has not been giving a strong band in western blot.
Application: Western blot
Primary: 1/1000
Block: milk
Sample: mouse brain homogenate, neuronal cell lysate, 40 ug
Results: Very weak band at 25 kDa

Read More
Answer

Thank you for contacting Abcam.

I am sorry that ab109236 is not producing a strong 25 kDa band in Western blot for your mouse brain homogenate. As we discussed on the phone, I am sending you a positive control free of charge. I also recommend attempting a primary dilution of 1/500 in case your samples simply contain a low amount of Neurogenin 2. Protease and phosphatase inhibitors should also be added if you have not already done so. Additionally, since the Neurogenin 2 protein is localized to the nucleus we recommend using RIPA buffer.

Our western blot protocol along with troubleshooting tips is given through the link below:
https://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

We initially discussed sending a HepG2 (liver carcinoma) cell lysate as the positive control, but after doing further searching I found a U-87 MG Nuclear Lysate (ab14903: human glioblastoma-astrocytoma) that has been used as a positive control for Neurogenin 2 and is more relavant to your research being a brain cell line as you desired.

Your order # is **** for ab14903, and will ship Monday for Tuesday delivery.

Please do not hesitate to contact us if you need any more advice or information, the positive control will tell us if the antibody is properly detected its antigen or not.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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