Anti-Neurofilament heavy polypeptide 抗体 [RNF402] (ab3966)

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Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)

    Key features and details

    • Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide
    • Suitable for: IHC-Fr
    • Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Monkey
    • Isotype: IgM

    製品の概要

    • 製品名

      Anti-Neurofilament heavy polypeptide antibody [RNF402]
      Neurofilament heavy polypeptide 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide

    • 由来種

      Mouse

    • 特異性

      Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms.

    • アプリケーション

      適用あり: IHC-Frmore details

    • 種交差性

      交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Monkey

    • 免疫原

      Tissue, cells or virus corresponding to Cow Neurofilament heavy polypeptide. Neurofilament preparation of calf brain tissue.
      Database link: P12036

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3966 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-Fr 1/10 - 1/100.

    ターゲット情報

    • 機能

      Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

    • 関連疾患

      Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

    • 配列類似性

      Belongs to the intermediate filament family.

    • 翻訳後修飾

      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)
      Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)

      Immunohistochemistry on frozen section of rat brain labeling Neurofilament heavy polypeptide with ab3966.

    参考文献 (5)

    ab3966 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3966 は 5 報の論文で使用されています。

    • Kim J  et al. Nerve Repair and Orthodromic and Antidromic Nerve Grafts: An Experimental Comparative Study in Rabbit. Biomed Res Int 2020:5046832 (2020). PubMed: 31998792
    • Han S  et al. Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine. Sci China Life Sci 61:2-13 (2018). PubMed: 28527111
    • Xiao Z  et al. One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59:647-55 (2016). PubMed: 27333785
    • Han S  et al. The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord 52:867-73 (2014). PubMed: 25311846
    • Haro H  et al. Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs. Spine J N/A:N/A (2013). Human . PubMed: 24295797

    レビューと Q&A

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    Question

    The isotype is IgM - what is the allotype?

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    Answer

    Thank you for your enquiry. This antibody is IgM kappa, we don't have any additional information. If you have any additional questions, please contact us again.

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