Anti-Neurofilament heavy polypeptide 抗体 [RNF402] (ab3966)

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Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)

    Key features and details

    • Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide
    • Suitable for: IHC-Fr
    • Reacts with: Rat
    • Isotype: IgM

    製品の概要

    • 製品名

      Anti-Neurofilament heavy polypeptide antibody [RNF402]
      Neurofilament heavy polypeptide 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide

    • 由来種

      Mouse

    • 特異性

      Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms.

    • アプリケーション

      適用あり: IHC-Frmore details

    • 種交差性

      交差種: Rat

    • 免疫原

      Tissue, cells or virus corresponding to Cow Neurofilament heavy polypeptide. Neurofilament preparation of calf brain tissue.
      Database link: P12036

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3966の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-Fr
    1/10 - 1/100.
    特記事項
    IHC-Fr
    1/10 - 1/100.

    ターゲット情報

    • 機能

      Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

    • 関連疾患

      Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

    • 配列類似性

      Belongs to the intermediate filament family.

    • 翻訳後修飾

      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 200 kDa neurofilament protein antibody
        • CMT2CC antibody
        • Nefh antibody
        • Neurofilament heavy polypeptide 200kDa antibody
        • Neurofilament heavy polypeptide antibody
        • Neurofilament triplet H protein antibody
        • NF H antibody
        • NF-H antibody
        • NFH antibody
        • NFH_HUMAN antibody
        see all

      画像

      • Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)
        Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)

        Immunohistochemistry on frozen section of rat brain labeling Neurofilament heavy polypeptide with ab3966.

      参考文献 (5)

      ab3966 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3966 は 5 報の論文で使用されています。

      • Kim J  et al. Nerve Repair and Orthodromic and Antidromic Nerve Grafts: An Experimental Comparative Study in Rabbit. Biomed Res Int 2020:5046832 (2020). PubMed: 31998792
      • Han S  et al. Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine. Sci China Life Sci 61:2-13 (2018). PubMed: 28527111
      • Xiao Z  et al. One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59:647-55 (2016). PubMed: 27333785
      • Han S  et al. The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord 52:867-73 (2014). PubMed: 25311846
      • Haro H  et al. Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs. Spine J N/A:N/A (2013). Human . PubMed: 24295797

      レビューと Q&A

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      Question

      The isotype is IgM - what is the allotype?

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      Answer

      Thank you for your enquiry. This antibody is IgM kappa, we don't have any additional information. If you have any additional questions, please contact us again.

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