Anti-Neurofilament heavy polypeptide 抗体 (ab8135)
Key features and details
- Rabbit polyclonal to Neurofilament heavy polypeptide
- Suitable for: IHC-FrFl, IHC-P, WB
- Reacts with: Mouse, Rat, Cow, Cat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-Neurofilament heavy polypeptide antibody
Neurofilament heavy polypeptide 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neurofilament heavy polypeptide -
由来種
Rabbit -
特異性
The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa). -
アプリケーション
適用あり: IHC-FrFl, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Cat, Human, Pig -
免疫原
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.03% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab8135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FrFl | 1/5000. | |
| IHC-P | 1/1000. | |
| WB | 1/10000. |
ターゲット情報
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機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
関連疾患
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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参照データベース
- Entrez Gene: 528842 Cow
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 100156492 Pig
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
see all -
別名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
画像
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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.
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Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody (ab8135)The image showing results obtained after protease pretreatment, illustrating high definition neurofilament staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
プロトコール
参考文献 (60)
ab8135 は 60 報の論文で使用されています。
- Grill M et al. Cellular localization and regulation of receptors and enzymes of the endocannabinoid system in intestinal and systemic inflammation. Histochem Cell Biol 151:5-20 (2019). PubMed: 30196316
- Zhang Q et al. The interleukin-4/PPAR? signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury. PLoS Biol 17:e3000330 (2019). PubMed: 31226122
- Shah S et al. Nitrosative Stress Is Associated with Dopaminergic Dysfunction in the HIV-1 Transgenic Rat. Am J Pathol 189:1375-1385 (2019). PubMed: 31230667
- Raimondo TM et al. Combined delivery of VEGF and IGF-1 promotes functional innervation in mice and improves muscle transplantation in rabbits. Biomaterials 216:119246 (2019). PubMed: 31203034
- Rodriguez CM et al. Translation of upstream open reading frames in a model of neuronal differentiation. BMC Genomics 20:391 (2019). PubMed: 31109297
