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  1. Link

    neurofilament-heavy-polypeptide-antibody-ab8135.pdf

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Anti-Neurofilament heavy polypeptide 抗体 (ab8135)

  • Datasheet
  • SDS
Reviews (25)Q&A (3)References (94)

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Abpromise

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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)
  • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)

Key features and details

  • Rabbit polyclonal to Neurofilament heavy polypeptide
  • Suitable for: IHC-FrFl, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Neurofilament heavy polypeptide antibody
    Neurofilament heavy polypeptide 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Neurofilament heavy polypeptide
  • 由来種

    Rabbit
  • 特異性

    The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).
  • アプリケーション

    適用あり: IHC-FrFl, WBmore details
  • 種交差性

    交差種: Mouse, Rat
  • 免疫原

    Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
    Database link: F1MSQ6

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.03% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Neurofilament heavy polypeptide protein (ab158986)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8135の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FrFl (1)
1/5000.
WB (2)
1/10000.
特記事項
IHC-FrFl
1/5000.
WB
1/10000.

ターゲット情報

  • 機能

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 関連疾患

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 配列類似性

    Belongs to the intermediate filament family.
  • 翻訳後修飾

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Target information above from: UniProt accession P12036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 380684 Mouse
    • Entrez Gene: 24587 Rat
    • SwissProt: P19246 Mouse
    • SwissProt: P16884 Rat
    • Unigene: 298283 Mouse
    • Unigene: 108194 Rat
    • 別名

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    画像

    • Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)
      Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)

      Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

      Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.

    • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)
      Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)
      All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution

      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat spinal cord tissue lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : Mouse spinal cord tissue lysate

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (94)

    ab8135 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8135 は 94 報の論文で使用されています。

    • Li H  et al. Local continuous glial cell derived neurotrophic factor release using osmotic pump promotes parasympathetic nerve rehabilitation in an animal model of cavernous nerve injury induced erectile dysfunction. Transl Androl Urol 10:258-271 (2021). PubMed: 33532315
    • Manousi A  et al. Identification of novel myelin repair drugs by modulation of oligodendroglial differentiation competence. EBioMedicine 65:103276 (2021). PubMed: 33714029
    • Liu H  et al. SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-?B signaling. Theranostics 11:4187-4206 (2021). PubMed: 33754056
    • Feng Y  et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923
    • Mughrabi IT  et al. Development and characterization of a chronic implant mouse model for vagus nerve stimulation. Elife 10:N/A (2021). PubMed: 33821789
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 28 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Sciatic nerve)
    Specification
    Sciatic nerve
    Read More

    MS. Lolita Singh

    Verified customer

    投稿 Nov 20 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    MR. Victor Bernal

    Verified customer

    投稿 Aug 26 2020

    Immunohistochemistry free floating abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Read More

    Mr. Daniel Lustberg

    Verified customer

    投稿 Aug 01 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Differentiated iPS cells)
    Permeabilization
    Yes - 0.3% Triton + 0.1% BSA
    Specification
    Differentiated iPS cells
    Blocking step
    0.1% BSA/10%FCS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 03 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate ph 6
    Permeabilization
    No
    Specification
    brain tissue
    Blocking step
    Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Herr Dr. Markus Kipp

    Verified customer

    投稿 Sep 07 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Skin\whiskers)
    Permeabilization
    No
    Specification
    Skin\whiskers
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    MR. Elena Ezhkova

    Verified customer

    投稿 Jan 09 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Nerve)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: ProK
    Permeabilization
    No
    Specification
    Nerve
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 11 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 05 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 05 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (engineered nervous tissue)
    Permeabilization
    No
    Specification
    engineered nervous tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 22 2014

    1-10 of 28 Abreviews or Q&A

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