アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    neurofilament-heavy-polypeptide-antibody-ab4680.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Other
Share by email

Anti-Neurofilament heavy polypeptide 抗体 (ab4680)

  • Datasheet
  • SDS
Reviews (12)Q&A (1)References (96)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

Key features and details

  • Chicken polyclonal to Neurofilament heavy polypeptide
  • Suitable for: ICC, IHC-FrFl, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgY

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Neurofilament heavy polypeptide antibody
    Neurofilament heavy polypeptide 一次抗体 製品一覧
  • 製品の詳細

    Chicken polyclonal to Neurofilament heavy polypeptide
  • 由来種

    Chicken
  • アプリケーション

    適用あり: ICC, IHC-FrFl, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー

    Preservative: 0.032% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    IgY fraction
  • 特記事項(精製)

    This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgY
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development

関連製品

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human Neurofilament heavy polypeptide protein (ab158986)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4680の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (1)
Use at an assay dependent concentration.
IHC-FrFl (1)
Use at an assay dependent concentration.
WB
1/1e+006. Predicted molecular weight: 200-220 kDa.
特記事項
ICC
Use at an assay dependent concentration.
IHC-FrFl
Use at an assay dependent concentration.
WB
1/1e+006. Predicted molecular weight: 200-220 kDa.

ターゲット情報

  • 機能

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 関連疾患

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 配列類似性

    Belongs to the intermediate filament family.
  • 翻訳後修飾

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Target information above from: UniProt accession P12036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 528842 Cow
    • Entrez Gene: 4744 Human
    • Entrez Gene: 380684 Mouse
    • Entrez Gene: 24587 Rat
    • Omim: 162230 Human
    • SwissProt: P12036 Human
    • SwissProt: P19246 Mouse
    • SwissProt: P16884 Rat
    • Unigene: 198760 Human
    • Unigene: 298283 Mouse
    • Unigene: 108194 Rat
    see all
  • 別名

    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

画像

  • Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)

    Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

    Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.

  • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution

    Lane 1 : Rat spinal cord lysate
    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Cow spinal cord lysate

    Predicted band size: 200-220 kDa

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

    Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

    Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section). 

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048

    ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.

プロトコール

  • Immunostaining of Cells in Tissue Culture

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (96)

ab4680 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4680 は 96 報の論文で使用されています。

  • Hosoya M  et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
  • Ewan EE  et al. Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism. Sci Rep 11:374 (2021). PubMed: 33431991
  • Jiang Y  et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
  • Triplet EM  et al. The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury. Neurobiol Dis 152:105294 (2021). PubMed: 33549720
  • Andreassi C  et al. Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity. Cell Rep 34:108778 (2021). PubMed: 33626357
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 13 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Read More

Abcam user community

Verified customer

投稿 Oct 09 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (BRAIN)
Antigen retrieval step
None
Permeabilization
Yes - 1%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Feb 18 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
None
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Mar 22 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Permeabilization
No
Specification
spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Read More

MR. Tamas Bellak

Verified customer

投稿 Mar 19 2018

Immunocytochemistry abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Differentiating Neuronal cells)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Differentiating Neuronal cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 17 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Pig Cell (Dorsal root ganglion neuron)
Specification
Dorsal root ganglion neuron
Permeabilization
Yes - Triton X
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 20 2015

Immunohistochemistry (Frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (dorsal root ganglian)
Specification
dorsal root ganglian
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 16 2015

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
mix BSA 1% and Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (BRAIN)
Specification
BRAIN
Permeabilization
Yes - Triton 2%
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Apr 30 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA1% + Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Aplysia Tissue sections (buccal ganglia)
Specification
buccal ganglia
Permeabilization
Yes - triton 2%
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Sep 30 2013

IHC - Wholemount abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (never)
Specification
never
Read More

DR. Xinpeng Dun

Verified customer

投稿 Jul 05 2012

1-10 of 13 Abreviews or Q&A

  •  戻る
  • 1
  • 2
  • 次へ 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.