Anti-Neurofilament heavy polypeptide 抗体 (ab4680)
Key features and details
- Chicken polyclonal to Neurofilament heavy polypeptide
- Suitable for: ICC, IHC-FrFl, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgY
製品の概要
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製品名
Anti-Neurofilament heavy polypeptide antibody
Neurofilament heavy polypeptide 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Neurofilament heavy polypeptide -
由来種
Chicken -
アプリケーション
適用あり: ICC, IHC-FrFl, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Full length native protein (cow intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell).
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
IgY fraction -
特記事項（精製）
This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab4680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-FrFl | Use at an assay dependent concentration. | |
| WB | 1/1e+006. Predicted molecular weight: 200-220 kDa. |
ターゲット情報
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機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
関連疾患
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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参照データベース
- Entrez Gene: 528842 Cow
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
- SwissProt: P16884 Rat
see all -
別名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
画像
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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.
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Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution
Lane 1 : Rat spinal cord lysate
Lane 2 : Mouse spinal cord lysate
Lane 3 : Cow spinal cord lysate
Predicted band size: 200-220 kDa -
Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.
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Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section). -
Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).
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Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.
参考文献 (66)
ab4680 は 66 報の論文で使用されています。
- Steinbach RJ et al. A neonatal nonhuman primate model of gestational Zika virus infection with evidence of microencephaly, seizures and cardiomyopathy. PLoS One 15:e0227676 (2020). PubMed: 31935257
- Chen B et al. Dynamic expression of Slit1-3 and Robo1-2 in the mouse peripheral nervous system after injury. Neural Regen Res 15:948-958 (2020). PubMed: 31719262
- Karakas N et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
- Laug D et al. Nuclear factor I-A regulates diverse reactive astrocyte responses after CNS injury. J Clin Invest 129:4408-4418 (2019). PubMed: 31498149
- Chen B et al. Analysis of Schwann Cell Migration and Axon Regeneration Following Nerve Injury in the Sciatic Nerve Bridge. Front Mol Neurosci 12:308 (2019). PubMed: 31920539
