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Anti-Neurofilament heavy polypeptide 抗体 [3G3] (ab19386)

Submit a review Submit a question References (3)
Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
  • Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

Key features and details

  • Mouse monoclonal [3G3] to Neurofilament heavy polypeptide
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Neurofilament heavy polypeptide antibody [3G3]
    Neurofilament heavy polypeptide 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3G3] to Neurofilament heavy polypeptide

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Neurofilament heavy polypeptide aa 1-226 (C terminal).

  • ポジティブ･コントロール

    • ICC: Rat brain microsome. WB: SH-SY5Y cells, rat brain lysates. IHC-P: Rat and human brain tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab19386の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/100 - 1/1000. Predicted molecular weight: 112 kDa.
特記事項
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/100 - 1/1000. Predicted molecular weight: 112 kDa.

ターゲット情報

  • 機能

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

  • 関連疾患

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 翻訳後修飾

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      All lanes : Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386) at 1/500 dilution

      Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
      Lane 2 : Rat brain tissue lysates

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : HRP-conjugated secondary antibody

      Predicted band size: 112 kDa



      Results show a band at ~200 kDa.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      Immunohistochemistry analysis of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) labelling Neurofilament Heavy Chain with ab19386. Positive staining in the cytoplasm and nucleus (right).
      To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament Heavy Chain monoclonal antibody diluted in 3% BSA-PBS at 1/20 dilution overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

      Immunohistochemistry analysis of Neurofilament, Heavy chain showing staining in the cytoplasm and axons of formalin-fixed, paraffin-embedded human brain tissue (B) and magnified section (C) compared with an isotype control (A). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2). Tissues were probed with a Neurofilament, Heavy chain monoclonal antibody for 60 minutes at a dilution of 2 µg/mL and detection was performed using an HRP-conjugated detection system for 30 minutes followed by DAB staining.

    • Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)
      Immunofluorescence of neurofilament, heavy chain in rat cerebral cortex cultures in green.

    参考文献 (3)

    ab19386 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab19386 は 3 報の論文で使用されています。

    • Segura-Anaya E  et al. A novel histochemical method of simultaneous detection by a single- or double-immunofluorescence and Bielschowsky's silver staining in teased rat sciatic nerves. J Neurosci Methods 304:46-51 (2018). ICC/IF . PubMed: 29596858
    • Xing J  et al. Role of TNF-a in Regulating the Exercise Pressor Reflex in Rats With Femoral Artery Occlusion. Front Physiol 9:1461 (2018). PubMed: 30374312
    • Janmaat CJ  et al. Human Dermal Fibroblasts Demonstrate Positive Immunostaining for Neuron- and Glia- Specific Proteins. PLoS One 10:e0145235 (2015). ICC/IF ; Mouse, Human . PubMed: 26678612

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