Anti-Neurofibromin 抗体 (ab17963)
Key features and details
- Rabbit polyclonal to Neurofibromin
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Neurofibromin antibody
Neurofibromin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neurofibromin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: HeLa, 293T, TCMK-1 and NIH3T3 whole cell lysates. IP: HeLa whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17963の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 319 kDa.
|
| IP |
Use a concentration of 2 - 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 319 kDa. |
|
IP
Use a concentration of 2 - 10 µg/ml. |
ターゲット情報
-
機能
Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity. -
関連疾患
Defects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
配列類似性
Contains 1 CRAL-TRIO domain.
Contains 1 Ras-GAP domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 280877 Cow
- Entrez Gene: 4763 Human
- Entrez Gene: 18015 Mouse
- Entrez Gene: 24592 Rat
- Omim: 613113 Human
- SwissProt: P21359 Human
- SwissProt: Q04690 Mouse
- SwissProt: P97526 Rat
see all -
別名
- DKFZp686J1293 antibody
- FLJ21220 antibody
- Neurofibromatosis Noonan syndrome antibody
see all
画像
-
Western blot - Anti-Neurofibromin antibody (ab17963)All lanes : Anti-Neurofibromin antibody (ab17963) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate in NETN lysis buffer
Lane 2 : 293T whole cell lysate in NETN lysis buffer
Lane 3 : Jurkat whole cell lysate in NETN lysis buffer
Lane 4 : TCMK-1 whole cell lysate in NETN lysis buffer
Lane 5 : NIH3T3 whole cell lysate in NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 319 kDa
Exposure time: 75 seconds -
Immunoprecipitation - Anti-Neurofibromin antibody (ab17963)Whole cell lysate (0.5-1.0mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: IP using rabbit anti-Neurofibromin antibody.
Lane 2: IP using ab17963 at 6 µg per reaction.
Lane 3: Control IgG.For western blotting, ab17963 was used at 1 µg/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab17963 は 9 報の論文で使用されています。
- Casingal CR et al. Identification of FMRP target mRNAs in the developmental brain: FMRP might coordinate Ras/MAPK, Wnt/ß-catenin, and mTOR signaling during corticogenesis. Mol Brain 13:167 (2020). PubMed: 33323119
- Jia J et al. Activated androgen receptor accelerates angiogenesis in cutaneous neurofibroma by regulating VEGFA transcription. Int J Oncol 55:157-166 (2019). PubMed: 31059067
- Moutal A et al. CRMP2-Neurofibromin Interface Drives NF1-related Pain. Neuroscience 381:79-90 (2018). IF . PubMed: 29655575
- Li X et al. Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-coupled Affinity Purification/Mass Spectrometry Analysis Revealed a Novel Role of Neurofibromin in mTOR Signaling. Mol Cell Proteomics 16:594-607 (2017). PubMed: 28174230
- Moutal A et al. Dissecting the role of the CRMP2-neurofibromin complex on pain behaviors. Pain 158:2203-2221 (2017). PubMed: 28767512
- Moutal A et al. CRISPR/Cas9 editing of Nf1 gene identifies CRMP2 as a therapeutic target in neurofibromatosis type 1-related pain that is reversed by (S)-Lacosamide. Pain 158:2301-2319 (2017). PubMed: 28809766
- Yzaguirre AD et al. Loss of neurofibromin Ras-GAP activity enhances the formation of cardiac blood islands in murine embryos. Elife 4:e07780 (2015). WB . PubMed: 26460546
- Shin J et al. Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Dis Model Mech 5:881-94 (2012). WB ; Zebrafish . PubMed: 22773753
- Hensley K et al. Proteomic identification of binding partners for the brain metabolite lanthionine ketimine (LK) and documentation of LK effects on microglia and motoneuron cell cultures. J Neurosci 30:2979-88 (2010). WB . PubMed: 20181595