Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (mouse N2A cell line)
Specification
mouse N2A cell line
Treatment
Transfected with NeuroD2 expressing vector
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 5% milk powder, 0.1% Tween-20 in TBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
pico ECL
Bands
Specific: 50 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Transfection of backbone vector only.
Positive control
Transfection of NeuroD2 expressing plasmid
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 13 2014