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リコンビナントRabMAb

Anti-NeuroD2 抗体 [EPR5135] (ab109406)

Reviews (3)Q&A (2)References (8)
Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
  • Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
  • Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
  • Anti-NeuroD2 antibody [EPR5135] (ab109406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5135] to NeuroD2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NeuroD2 antibody [EPR5135]
    NeuroD2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5135] to NeuroD2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human cerebellum, Human hippocampus, and fetal brain, Mouse cerebellum, Rat cerebellum lysates

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109406の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

画像

  • Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
    Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
    All lanes : Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution (Purified)

    Lane 1 : Mouse cerebellum lysates
    Lane 2 : Rat cerebellum lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
    Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
    All lanes : Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution (Purified)

    Lane 1 : Human cerebellum lysates at 20 µg
    Lane 2 : Human fetal brain lysates at 20 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
    Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406)
    All lanes : Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human hippocampus lysate
    Lane 3 : Human fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?

  • Anti-NeuroD2 antibody [EPR5135] (ab109406)
    Anti-NeuroD2 antibody [EPR5135] (ab109406)

参考文献 (8)

ab109406 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109406 は 8 報の論文で使用されています。

  • Lipinski M  et al. KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain. Nat Commun 11:2588 (2020). PubMed: 32444594
  • Guzelsoy G  et al. Terminal neuron localization to the upper cortical plate is controlled by the transcription factor NEUROD2. Sci Rep 9:19697 (2019). PubMed: 31873146
  • van Weert LTCM  et al. Mechanistic Insights in NeuroD Potentiation of Mineralocorticoid Receptor Signaling. Int J Mol Sci 20:N/A (2019). PubMed: 30934833
  • Peter CJ  et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun 10:4112 (2019). PubMed: 31511512
  • Hahn MA  et al. Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation. Sci Adv 5:eaax0080 (2019). PubMed: 31681843
  • Bayam E  et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . PubMed: 26341353
  • Katsushima K  et al. Contribution of MicroRNA-1275 to Claudin11 Protein Suppression via a Polycomb-mediated Silencing Mechanism in Human Glioma Stem-like Cells. J Biol Chem 287:27396-406 (2012). WB ; Human . PubMed: 22736761
  • Guner G  et al. NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons. eNeuro 4:N/A (0). PubMed: 28303257

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Immunoprecipitation abreview for Anti-NeuroD2 [EPR5135] antibody - ChIP Grade

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Mouse Cell lysate - whole cell (Neuro2A cell line)
Specification
Neuro2A cell line
Treatment
Transfected with NeuroD2-myc or emtpty vector pcDNA4
Total protein in input
200 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 11 2014

Western blot abreview for Anti-NeuroD2 [EPR5135] antibody - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (mouse N2A cell line)
Specification
mouse N2A cell line
Treatment
Transfected with NeuroD2 expressing vector
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 13 2014

ChIP abreview for Anti-NeuroD2 [EPR5135] antibody - ChIP Grade

 Excellent
Abreviews
abreview image
Application
ChIP
Detection step
Other
Sample
Mouse Tissue lysate - whole (Cerebral cortex tissue from postnatal day 0 mouse)
Specification
Cerebral cortex tissue from postnatal day 0 mouse
Negative control
ChIP performed with unrelated antibody (anti-GFP antibody).
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
Known target of NeuroD2, which is the promoter regions of Nhlh2 gene. Primer sequence for PCR was: Nhlh2_F CTCACGAACTTCACCCGCAC Nhlh2_R AAATTGACTCCTCGGCCCTC
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 18 2014

Question

Inquiry: Hello Abcam, I was wondering if you had a specific protocol that you could send to me for IHC-P uising the Rabbit monoclonal to NeuroD2? I used more than your recommended dilution, but did not get a result. Thank you.

Read More
Answer

According to our records, ab109406 was proving difficult to use and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

Read More

Question

Inquiry: Hello Abcam, I was wondering if you had a specific protocol that you could send to me for IHC-P uising the Rabbit monoclonal to NeuroD2? I used more than your recommended dilution, but did not get a result. Thank you

Read More
Answer

Thank you for contacting us and for letting us know about the trouble with ab109406.

I have attached the protocol that was used by the lab to test this antibody.

Could you tell me a bit more about the problem and the protocol used, and I'll see if there are any specific suggestions to make? What kind of tissue are you staining, and what methods have you tried?

I look forward to hearing from you. We do guarantee this antibody to work in IHC-P with mouse, rat, and human tissue for up to six months after purchase, so I will be happy to send a replacement or issue a credit or refund if necessary.

Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

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