Anti-NeuroD2 抗体 (ab104430)
Key features and details
- Rabbit polyclonal to NeuroD2
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NeuroD2 antibody
NeuroD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NeuroD2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Gorilla, Chinese hamster
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免疫原
Synthetic peptide corresponding to Mouse NeuroD2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab124135) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (4) |
Use at an assay dependent concentration.
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| IHC-Fr | (1) |
1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
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機能
Appears to mediate neuronal differentiation. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4761 Human
- Entrez Gene: 18013 Mouse
- Entrez Gene: 54276 Rat
- Omim: 601725 Human
- SwissProt: Q15784 Human
- SwissProt: Q62414 Mouse
- SwissProt: Q63689 Rat
- Unigene: 322431 Human
see all -
別名
- bHLHa1 antibody
- class A basic helix loop helix protein 1 antibody
- Class A basic helix-loop-helix protein 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD2 antibody (ab104430)
IHC image of NeuroD2 staining in a formalin fixed, paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104430, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-NeuroD2 antibody (ab104430)All lanes : Anti-NeuroD2 antibody (ab104430) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : P0 Mouse Brain Mouse Tissue Lysate
Lane 5 : P7 Mouse Brain Tissue Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466) with Immunising peptide at 1 µg/ml
Lane 7 : Cerebellum Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 8 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 9 : P0 Mouse Brain Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 10 : P7 Mouse Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD2 antibody (ab104430)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of NeuroD2 staining on mouse CA1 hippocampus sections using ab104430 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD2 antibody (ab104430)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of NeuroD2 staining on rat CA1 hippocampus sections using ab104430 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours -
Immunohistochemistry (Frozen sections) - Anti-NeuroD2 antibody (ab104430)ab104430 staining NeuroD2 in 10% formaldehyde perfusion fixed 6 week old frozen mouse brain section (dentate gyrus). No antigen retrieval was performed. The section was incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104430, 1/2000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab104430 は 14 報の論文で使用されています。
- Pellegrini L et al. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27:951-961.e5 (2020). PubMed: 33113348
- Yan W et al. MOUSE RETINAL CELL ATLAS: MOLECULAR IDENTIFICATION OF OVER SIXTY AMACRINE CELL TYPES. J Neurosci N/A:N/A (2020). PubMed: 32457074
- Fan X et al. Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development. Sci Adv 6:eaaz2978 (2020). PubMed: 32923614
- Lennox AL et al. Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development. Neuron 106:404-420.e8 (2020). PubMed: 32135084
- Bonnefont J et al. Cortical Neurogenesis Requires Bcl6-Mediated Transcriptional Repression of Multiple Self-Renewal-Promoting Extrinsic Pathways. Neuron 103:1096-1108.e4 (2019). PubMed: 31353074