Neuraminidase Activity Assay Kit (Fluorometric) (ab185436)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Serum, Suspension cells, Tissue
- Sensitivity: 2 mU/ml
製品の概要
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製品名
Neuraminidase Activity Assay Kit (Fluorometric)
Neuraminidase キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Serum, Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Enzyme activity (quantitative) -
検出感度
< 2 mU/ml -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam's Neuraminidase Activity Assay Kit (Fluorometric) (ab185436) provides a simple and sensitive method for measuring neuraminidase (NA) activity using fluorescence (Ex/Em = 530/590 nm). The assay utilizes NA Probe to detect the neuraminidase activity. This high-throughput adaptable assay kit can detect NA activity as low as 2.0 mU/mL in a variety of samples.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K732 Neuraminidase Activity Fluorometric Assay Kit. K732-100 is the same size as the 100 test size of ab185436.
Neuraminidase (NA) is a very common enzyme that hydrolyzes terminal sialic acid residues on polysaccharide chains; most often a galactose residue. NA activity plays a key role in the invasion of target cells and the replication of influenza virus. NA activity also assists in the elution of progeny viruses from infected cells, and prevents self-aggregation of virus. Thus, NA is an important target for drug development.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Galactose Standard (100nmol/ul) 1 x 0.1ml NA Assay Buffer 1 x 30ml NA Enzyme Mix I 1 vial NA Enzyme Mix II 1 vial NA Positive Control 1 x 0.1ml NA Probe 1 x 0.2ml NA Substrate 1 vial -
研究分野
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機能
Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage. -
組織特異性
Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain. -
関連疾患
Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells. -
配列類似性
Belongs to the glycosyl hydrolase 33 family.
Contains 4 BNR repeats. -
ドメイン
A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes. -
翻訳後修飾
N-glycosylated.
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane. -
細胞内局在
Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. - Information by UniProt
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別名
- Acetylneuraminyl hydrolase
- exo-alpha-sialidase
- G9 sialidase
see all
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185436 は論文での使用が確認できていません。