Biotin Anti-NeuN 抗体 [EPR12763] (ab204681)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR12763] to NeuN
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: Biotin
Related conjugates and formulations
製品の概要
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製品名
Biotin Anti-NeuN antibody [EPR12763]
NeuN 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit monoclonal [EPR12763] to NeuN -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset -
免疫原
Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: A6NFN3 -
ポジティブ・コントロール
- IHC-P: FFPE human, mouse and rat cerebellum (normal) tissue sections.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12763 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
- Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
- Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
- Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
- Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
- Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207282)
- Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
- FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab204681の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
RNA-binding protein that regulates alternative splicing events. -
配列類似性
Contains 1 RRM (RNA recognition motif) domain. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 101084840 Cat
- Entrez Gene: 608803 Dog
- Entrez Gene: 102186905 Goat
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- Entrez Gene: 101111982 Sheep
- Entrez Gene: 100538330 Zebrafish
see all -
別名
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
画像
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IHC image of NeuN staining in a section of formalin-fixed paraffin-embedded normal rat cerebellum, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab204681, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of NeuN staining in a section of formalin-fixed paraffin-embedded normal mouse cerebellum, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab204681, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of NeuN staining in a section of formalin-fixed paraffin-embedded human cerebellum (normal), performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin. The section was then incubated with ab204681, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab204681 は 1 報の論文で使用されています。
- Liao R et al. Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity. Front Cell Dev Biol 8:614624 (2020). PubMed: 33585449