Anti-Nestin 抗体 [EPR22023] (ab221660)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22023] to Nestin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
-
製品名
Anti-Nestin antibody [EPR22023]
Nestin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22023] to Nestin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Neuro-2a whole cell lysate; Mouse heart and P0 brain lysate. IHC-P: Mouse embryonic day 14.5 cerebrum, kidney and lung tissue. IHC-Fr: Mouse and rat kidney tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22023 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221660の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 140-250 kDa (predicted molecular weight: 177 kDa).
|
|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IHC-Fr |
1/100.
Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
特記事項 |
---|
WB
1/1000. Detects a band of approximately 140-250 kDa (predicted molecular weight: 177 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
ターゲット情報
-
機能
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
組織特異性
CNS stem cells. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
翻訳後修飾
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
-
参照データベース
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- SwissProt: P21263 Rat
- Unigene: 331129 Mouse
- Unigene: 9701 Rat
-
別名
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
画像
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of mouse kidney (PMID: 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit at a 1/1000 dilution.Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of rat kidney (PMID: 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit at a 1/1000 dilution.Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
All lanes : Anti-Nestin antibody [EPR22023] (ab221660) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 2 : Mouse heart lysate at 10 µg
Lane 3 : Mouse P0 brain lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 177 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1: 26 seconds; Lane 2: 81 seconds; Lane 3: 15 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 23319587, PMID: 23407796).
-
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in mouse lung (PMID: 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of glomerulus and stromal cells in mouse kidney (PMID: 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded mouse embryonic day 14.5 cerebrum tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on cerebrum of embryonic day 14.5 mouse (PMID: 16728475) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (11)
ab221660 は 11 報の論文で使用されています。
- Wei SW et al. Role of the hydrogen sulfide-releasing donor ADT-OH in the regulation of mammal neural precursor cells. J Cell Physiol 237:2877-2887 (2022). PubMed: 35342944
- Tang CF et al. Short-Chain Fatty Acids Ameliorate Depressive-like Behaviors of High Fructose-Fed Mice by Rescuing Hippocampal Neurogenesis Decline and Blood-Brain Barrier Damage. Nutrients 14:N/A (2022). PubMed: 35565849
- Fatania K et al. Exploratory Analysis of Serial 18F-fluciclovine PET-CT and Multiparametric MRI during Chemoradiation for Glioblastoma. Cancers (Basel) 14:N/A (2022). PubMed: 35884545
- Qian C et al. Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. Int J Oncol 61:N/A (2022). PubMed: 35929514
- Li L et al. FBXW7 mediates high glucose-induced SREBP-1 expression in renal tubular cells of diabetic nephropathy under PI3K/Akt pathway regulation. Mol Med Rep 23:N/A (2021). PubMed: 33537812