製品の概要

  • 製品名
    Anti-Nestin antibody [7A3]
    Nestin 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [7A3] to Nestin
  • 由来種
    Rat
  • アプリケーション
    適用あり: IHC-Fr, ICC/IFmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse E16 embryonic cerebral cortex extracts

  • ポジティブ・コントロール
    • Neural progenitor cells from mouse fetal brain.
  • 特記事項


    ab81462 is very useful for immunostaining of mouse embryonic brain. It has very low background in immunostaining using mouse tissues and is also useful for doubleb staining with mouse and rabbit antibodies.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • 組織特異性
      CNS stem cells.
    • 配列類似性
      Belongs to the intermediate filament family.
    • 発生段階
      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • 翻訳後修飾
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    画像

    • Primary culture of neural progenitor cells from mouse fetal brain stained with ab81462.
    • Primary culture of neural progenitor cells from mouse fetal brain, without staining (bright field image).

    参考文献

    This product has been referenced in:
    • Ngwenya LB  et al. Implantation of Neuronal Stem Cells Enhances Object Recognition without Increasing Neurogenesis after Lateral Fluid Percussion Injury in Mice. Stem Cells Int 2018:4209821 (2018). ICC/IF ; Mouse . Read more (PubMed: 29531536) »
    • Wlodarczyk A  et al. A novel microglial subset plays a key role in myelinogenesis in developing brain. EMBO J 36:3292-3308 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28963396) »

    See all 3 Publications for this product

    レビューと Q&A

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cor1 cells)
    Specification
    Cor1 cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% TWEEN
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 May 25 2011

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain and kidney)
    Specification
    Brain and kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Dec 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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