Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (hair follicle culture)
Specification
hair follicle culture
Permeabilization
Yes - methanol
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Diluent: PBS-T+1%BSA
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/500
Additional data
Additional Notes
The image shows nestin-postive cells migrating out of the cultured hair follicle.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Heiko Locher
Verified customer
投稿 Sep 23 2013