Anti-NEK7 抗体 [EPR4901] - BSA and Azide free (ab247860)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4901] to NEK7 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NEK7 antibody [EPR4901] - BSA and Azide free
NEK7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4901] to NEK7 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab247860 is the carrier-free version of ab109433.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4901 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247860の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 0.2 µg/ml.
PFA fixation not recommended |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 0.2 µg/ml. PFA fixation not recommended |
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
ターゲット情報
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組織特異性
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
配列類似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 140609 Human
- Entrez Gene: 59125 Mouse
- Entrez Gene: 360850 Rat
- Omim: 606848 Human
- SwissProt: Q8TDX7 Human
- SwissProt: Q9ES74 Mouse
- SwissProt: D3ZBE5 Rat
- Unigene: 24119 Human
see all -
別名
- EC 2.7.11.1 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
see all
画像
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Flow cytometry overlay histogram showing left wild-type HAP1 WT and right NEK7 knockout HAP1 WT stained with ab247860 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab109433) (1x 106 in 100µl at 0.2 µg/ml (1/3135 dilution)) for 30min at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30min at 22°C
Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] was used at the same concentration and conditions as the primary antibody (black line). Unlabelled sample was also used as a control (blue line).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
PFA fixation is not recommended.
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This data was developed using ab109433, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109433 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab109433 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. ab109433 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-NEK7 antibody [EPR4901] (ab109433) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal liver lysate
Lane 6 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDaThis data was developed using ab109433, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247860 は論文での使用が確認できていません。