Anti-NEK7 抗体 [EPR4901] (ab109433)
![Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)](https://www.abcam.co.jp/ps/products/109/ab109433/Images/ab109433-268424-anti-nek7-antibody-epr4901-western-blot.jpg "Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4901] to NEK7
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NEK7 antibody [EPR4901]
NEK7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4901] to NEK7 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human brain, HeLa, A549, HepG2, fetal liver, and Jurkat lysates; Jurkat cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4901 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109433の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 35 kDa.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 35 kDa. |
ターゲット情報
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組織特異性
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
配列類似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 140609 Human
- Entrez Gene: 59125 Mouse
- Entrez Gene: 360850 Rat
- Omim: 606848 Human
- SwissProt: Q8TDX7 Human
- SwissProt: Q9ES74 Mouse
- SwissProt: D3ZBE5 Rat
- Unigene: 24119 Human
see all -
別名
- EC 2.7.11.1 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
see all
画像
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Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109433 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.ab109433 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab109433 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)](https://www.abcam.co.jp/ps/products/109/ab109433/Images/ab109433-122270-anti-nek7-antibody-epr4901-western-blot.jpg)
Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)All lanes : Anti-NEK7 antibody [EPR4901] (ab109433) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal liver lysate
Lane 6 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
![Flow Cytometry (Intracellular) - Anti-NEK7 antibody [EPR4901] (ab109433)](https://www.abcam.co.jp/ps/products/109/ab109433/Images/ab109433-122269-anti-nek7-antibody-epr4901-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NEK7 antibody [EPR4901] (ab109433)Intracellular flow cytometric analysis of NEK7 in permeabilized Jurkat cells using ab109433 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-NEK7 antibody [EPR4901] (ab109433)](https://www.abcam.co.jp/ps/products/109/ab109433/Images/ab109433-4-benefits-of-recombinant-antibodies.png)
Anti-NEK7 antibody [EPR4901] (ab109433)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab109433 は 2 報の論文で使用されています。
- Perera AP et al. MCC950, a specific small molecule inhibitor of NLRP3 inflammasome attenuates colonic inflammation in spontaneous colitis mice. Sci Rep 8:8618 (2018). WB ; Mouse . PubMed: 29872077
- Xiong Y et al. Characteristics of peripheral and intrahepatic regulatory B cells in HBV-related liver cirrhosis. Int J Clin Exp Pathol 11:4545-4551 (2018). PubMed: 31949852