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Negative control siRNA vector (pGB control) (ab12505)

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Enzyme activity assay - Negative control siRNA vector (pGB control) (ab12505)
  • Vector - Negative control siRNA vector (pGB control) (ab12505)
  • Other
  • Other

製品の概要

  • 製品名

    Negative control siRNA vector (pGB control)
    siRNA Vector ベクター 製品一覧

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called 9500C Control siRNA Vector (pGB-control). 9500C-20 is the same size as the 20 µg size of ab12505.

    pGB expression vectors contain the human U6 RNA polymerase III promoter, which directs constitutive, high-level expression of short RNA transcripts in many cells. Each vector also contains the neomycin/kanamycin-resistance gene to provide kanamycin resistance in bacteria and the G418 resistance in mammalian cells. The pGB Negative Control vector contains a insert that does not have significant homology to mammalian genes expressed in human, mouse, and rat, and it can be used as a negative control for pGB-Caspase siRNA vectors.

    This siRNA negative control vector is used as a negative control for siRNA expression. The pGB siRNA vectors can be transfected into mammalian cells using Lipofectamine (Invitrogen). For transient transfection, cells can be analyzed in 24-96 hours following transfections, by Western blot analysis or other detection means. For stable transfections, cells can be selected in G418 selection medium to obtain stable cell lines with the specific gene blocked.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
  • Concentration information loading...

  • 研究分野

  • 関連性

    Small interfering RNAs (siRNAs) are short, double-stranded RNA molecules that can target and degrade specific complementary mRNAs. The target gene-specific degradation is an effective means of gene suppression. Abcam's pGB siRNA vectors are designed to provide efficient, long-term suppression of a target gene in cultured mammalian cells and in vivo. The pGB vectors have been optimized for suppressing expression of target genes in mammalian cells by using the human U6 promotor (a RNA polymerase III promotor) which generates large amounts of siRNA in mammalian cells. The pGB expression vector also provides neomycin resistance marker for the selection of stable cell lines, permitting long-term suppression of the target gene. Validation: The antibodies listed as related products have not been previously used by Abcam to validate this vector. The siRNA vectors have been tested in HeLa and/or 293 cell lines. The siRNA-mediated knockdown was analyzed by Western blot.

  • 別名

    • siRNA vector
    • Small interfering ribose nucleic acid vector
    • Small interfering RNA vector

関連製品

画像

  • Enzyme activity assay - Negative control siRNA vector (pGB control) (ab12505)
    Enzyme activity assay - Negative control siRNA vector (pGB control) (ab12505)

    RNA Interference Mechanism

    Once processed, the vector expressed siRNA is incorporated into a multi-protein nuclease complex known as the RNA-induced silencing complex (RISC). Homology between the sense portion of the siRNA sequence and the mRNA enables the nuclease enzyme to bind and cleave the caspase transcript into small pieces, which are degraded by the cell's machinery.

  • Vector - Negative control siRNA vector (pGB control) (ab12505)
    Vector - Negative control siRNA vector (pGB control) (ab12505)

    pGB vector

    Features and Positions:

    Human U6 Promoter: 1-256
    Multiple cloning Site: 259-285
    3' Primer: 398-426 (GAAGCATTTATCAGGGTTATTGTCTCATG)
    SV40 Promoter: 470-808
    Neomycin / Kanamycin Resistance ORF: 843-1634
    5' Primer: 2789-2813 (CGTCGATTTTTGTGATGCTCGTCAG)
    pUC Origin of Replication: 2222-3003

  • Other
    Other

    RNA Interference Mechanism

    Once processed, the vector expressed siRNA is incorporated into a multi-protein nuclease complex known as the RNA-induced silencing complex (RISC). Homology between the sense portion of the siRNA sequence and the mRNA enables the nuclease enzyme to bind and cleave the caspase transcript into small pieces, which are degraded by the cell's machinery.

  • Other
    Other

    pGB vector

    Features and Positions:

    Human U6 Promoter: 1-256
    Multiple cloning Site: 259-285
    3' Primer: 398-426 (GAAGCATTTATCAGGGTTATTGTCTCATG)
    SV40 Promoter: 470-808
    Neomycin / Kanamycin Resistance ORF: 843-1634
    5' Primer: 2789-2813 (CGTCGATTTTTGTGATGCTCGTCAG)
    pUC Origin of Replication: 2222-3003

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab12505 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab12505 は論文での使用が確認できていません。

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