Anti-Nectin 3 抗体 [103-A1] (ab16913)
Key features and details
- Rat monoclonal [103-A1] to Nectin 3
- Suitable for: IHC-Fr, IP, ICC/IF, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-Nectin 3 antibody [103-A1]
Nectin 3 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [103-A1] to Nectin 3 -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, IP, ICC/IF, Flow Cyt, Functional Studiesmore details
適用なし: WB -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Recombinant fragment, corresponding to Mouse Nectin 3 extracellular domain, amino acids 50-400 fused to IgG Fc.
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ポジティブ･コントロール
- L-cells (A cultured line of C3H mouse fibroblasts)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Provided as a 0.2µm purified antibody solution -
ポリ/モノ
モノクローナル -
クローン名
103-A1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16913の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
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| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Functional Studies |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
Functional Studies
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. -
組織特異性
Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. -
配列類似性
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 58998 Mouse
- SwissProt: Q9JLB9 Mouse
- Unigene: 328072 Mouse
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別名
- CD113 antibody
- CDw113 antibody
- DKFZP566B0846 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab16913 は 4 報の論文で使用されています。
- Lachke SA et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet 131:235-50 (2012). IHC-Fr ; Mouse . PubMed: 21769484
- Lin YW et al. Localization of ubiquitin specific protease 26 at blood-testis barrier and near Sertoli cell-germ cell interface in mouse testes. Int J Androl 34:e368-77 (2011). PubMed: 21449984
- Tanaka-Okamoto M et al. Increased susceptibility to spontaneous lung cancer in mice lacking LIM-domain only 7. Cancer Sci 100:608-16 (2009). PubMed: 19215226
- Toyama Y et al. Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies. Asian J Androl 10:577-84 (2008). IHC-P ; Mouse . PubMed: 18097512
