Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12)
Total protein in input
500 µg
Immuno-precipitation step
Protein G
Specification
PC12
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA Buffer
Concentration
4 µg/mg lysate
Western Blot antibody
Name
Abcam antibody:Anti-NEAS antibody [D8B7]
Additional data
Additional Notes
This antibody works nicely in IP/IB experiments. I would recommend using this antibody at 1 in 2000 or less when blotting back. The 150 kDa doublet could be a degradation product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 30 2007