アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    ncx1-antibody-c2c12-ab2869.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Calcium Signaling Calcium Channels
Share by email

Anti-NCX1 抗体 [C2C12] (ab2869)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (28)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
  • Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

Key features and details

  • Mouse monoclonal [C2C12] to NCX1
  • Suitable for: IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

こちらの製品もご検討ください

アッセイ
Product image
Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric) (ab112119)
一次抗体
Product image
Anti-BIN1 antibody [EPR13463-25] (ab185950)
一次抗体
Product image
Anti-Phospholamban antibody (ab85146)

関連製品

製品の概要

  • 製品名

    Anti-NCX1 antibody [C2C12]
    NCX1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [C2C12] to NCX1
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Dog NCX1. Purified from canine cardiac sodium/calcium exchanger.

  • エピトープ

    This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.
  • ポジティブ・コントロール

    • IHC-P: Normal human kidney tissue. IHC-Fr: Normal human kidney tissue
  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 一次抗体 備考

    The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.
  • ポリ/モノ

    モノクローナル
  • クローン名

    C2C12
  • アイソタイプ

    IgM
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (ab47827)
    • Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2869の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr (1)
Use a concentration of 1 µg/ml.

PubMed: 21408028

Flow Cyt
1/20 - 1/100.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use a concentration of 1 µg/ml.

PubMed: 21408028

Flow Cyt
1/20 - 1/100.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.
  • 組織特異性

    Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung.
  • 配列類似性

    Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily.
    Contains 2 Calx-beta domains.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession P32418 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6546 Human
    • Entrez Gene: 20541 Mouse
    • Entrez Gene: 397365 Pig
    • Entrez Gene: 100328570 Rabbit
    • Entrez Gene: 29715 Rat
    • Omim: 182305 Human
    • SwissProt: P23685 Dog
    • SwissProt: P48766 Guinea pig
    • SwissProt: P32418 Human
    • SwissProt: P70414 Mouse
    • SwissProt: Q01728 Rat
    • Unigene: 31961 Human
    • Unigene: 468274 Human
    • Unigene: 728911 Human
    • Unigene: 265834 Mouse
    • Unigene: 118972 Rat
    see all
  • 別名

    • CNC antibody
    • DKFZp779F0871 antibody
    • MGC119581 antibody
    • Na(+)/Ca(2+)-exchange protein 1 antibody
    • Na+/Ca2+ exchange protein 1 antibody
    • Na+/Ca2+ exchanger antibody
    • NAC1_HUMAN antibody
    • NCX 1 antibody
    • NCX antibody
    • NCX1 antibody
    • SLC8A1 antibody
    • SLC8A1 protein antibody
    • Sodium Calcium Exchanger antibody
    • Sodium/calcium exchanger 1 antibody
    • Solute carrier family 8 (sodium/calcium exchanger) member 1 antibody
    • Solute carrier family 8 member 1 antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)

    IHC image of NCX1 staining in a section of frozen normal human kidney*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab2869 at 1µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

    IHC image of NCX1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2869, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)
    Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)

    Overlay histogram showing HEK293 cells stained with ab2869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2869, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)This image is courtesy of an anonymous Abreview
    ab2869 staining NCX1 in Human kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% normal goat serum in 1XPBS + 0.05% Tween20 for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. Ab47827 (1/500) was used as the secondary antibody.

    See Abreview

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (28)

ab2869 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2869 は 28 報の論文で使用されています。

  • Wu X  et al. Seipin Deficiency Accelerates Heart Failure Due to Calcium Handling Abnormalities and Endoplasmic Reticulum Stress in Mice. Front Cardiovasc Med 8:644128 (2021). PubMed: 33778025
  • Hanan M  et al. A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress. EMBO Mol Med 12:e11942 (2020). PubMed: 32715657
  • Gao H & Zhou Z Effect of Xylo-Oligosaccharides Supplementation by Drinking Water on the Bone Properties and Related Calcium Transporters in Growing Mice. Nutrients 12:N/A (2020). PubMed: 33228037
  • Sun F  et al. Detrimental Effect of C-Reactive Protein on the Cardiometabolic Cells and Its Rectifying by Metabolic Surgery in Obese Diabetic Patients. Diabetes Metab Syndr Obes 13:1349-1358 (2020). PubMed: 32425567
  • Yang HY  et al. The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes. J Cell Mol Med 24:6762-6772 (2020). PubMed: 32342656
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 15 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (coronary artery, heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6)
Permeabilization
No
Specification
coronary artery, heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 27 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Nov 04 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCX1 antibody [C2C12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal Kidney)
Specification
Normal Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization
Yes - 0.05% Tween20
Blocking step
Normal Goat Serum in 1X PBS (0.05% Tween20) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 20 2010

Question

Our customer retested following your recommendation but the band still did not show in positive control (Hek293 cells).
She said that secondary antibody has been used for WB without any problem.
Please check attached file.

Best regards,

Read More

Abcam community

Verified customer

Asked on Jun 28 2012

Answer

Thank you and your customerfor taking the time totry the tipsand contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time your customerhas spent in the laboratory and understandtheir concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest your customerhas regrettably received a bad vial.

I apologise for the inconvenience and am pleased to offer your customera free of charge replacement (also possible with an alternative antibody) or credit note in compensation.

In addition, I can suggest your customer may like to consider the following suggestions for future experiments:

Thank you and your customerfor your cooperation. I look forward to hearing from you with details of how your customerwould like to proceed.

Read More

Abcam Scientific Support

Answered on Jun 28 2012

Question

Product code: 2869
Lot number: GR37744-4

Inquiry: Our customer would like to know what size detected in human sample. Please let me know.

EA hy926 cell

Read More

Abcam community

Verified customer

Asked on Jun 22 2012

Answer

Thank you and your customer for your inquiry.

I can confirm that ab2869 detects a 120 kDa protein representing the sodium / calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. The human protein shows the same molecular weight and I assume that bands at 70 kDa and 160 kDa also present a proteolytic fragment and anon-reduced exchanger respectively.

This publications shows the molecular weight of human NCX1:

Liu J et al. Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation. Stem Cells 25:3038-44 (2007).

This publication also uses milk for blocking and a over night incubation of ab2869.

I therefore recommend to following:

1. ) I suggest to use a positive control. Hek293 cells and human kidney are shown to be NCX1 positive.

2.) I can also suggest to check if the secondary antibody works.

3.) NCX1 is a multi pass protein and I therefore do recommend not to boil the samples but only to heat them up to 70C for 10 minutes.

I hope this information is helpful. please do not hesitate to contactme again with any further questions in this matter.

Read More

Abcam Scientific Support

Answered on Jun 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.