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Anti-NCR1 抗体 [n1D9] (ab14823)

Submit a review Q&A (1)References (3)
Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)
  • Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)
  • Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)

Key features and details

  • Mouse monoclonal [n1D9] to NCR1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-NCR1 antibody [n1D9]
    NCR1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [n1D9] to NCR1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein, corresponding to amino acids 22-255 of Human NCR1

  • ポジティブ･コントロール

    • Flow Cyt: Human peripheral blood mononuclear cells or natural killer cells, PBMC cells, HeLa cells.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 10% Glycerol
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • 特記事項（精製）

    Purified from TCS.

  • ポリ/モノ

    モノクローナル

  • クローン名

    n1D9

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14823の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

  • 組織特異性

    Selectively expressed by both resting and activated NK cells.

  • 配列類似性

    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.

  • 翻訳後修飾

    N-glycosylated.
    O-glycosylated.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activating NK receptor NK p46 antibody
      • CD335 antibody
      • FLJ99094 antibody
      • hNKp46 antibody
      • Ly94 antibody
      • Lymphocyte antigen 94 antibody
      • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
      • Lymphocyte antigen 94 homolog antibody
      • Natural cytotoxicity triggering receptor 1 antibody
      • Natural killer cell p46-related protein antibody
      • NCR1 antibody
      • NCT1 antibody
      • NCTR1_HUMAN antibody
      • NK cell activating receptor antibody
      • NK cell-activating receptor antibody
      • NK-p46 antibody
      • NKp46 antibody
      see all

    画像

    • Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)
      Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)

      Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with ab14823 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

    • Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)
      Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)

      Flow cytometry analysis of NKp46 in PBMC cells. The cell was stained with ab14823 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

    • Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)
      Flow Cytometry - Anti-NCR1 antibody [n1D9] (ab14823)

      ab14823 staining human PBMC by flow cytometry.

      This image was produced using the ascites version of this antibody. 

    参考文献 (3)

    ab14823 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14823 は 3 報の論文で使用されています。

    • Qu S  et al. Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model. Int J Cancer 143:419-429 (2018). PubMed: 29441566
    • Haberthur K  et al. NKG2D ligand expression in pediatric brain tumors. Cancer Biol Ther 17:1253-1265 (2016). PubMed: 27834580
    • Ascierto ML  et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. J Transl Med 11:145 (2013). IHC-P . PubMed: 23758773

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Inquiry: Hello, I would like to do a test for antibody specificity for the NCR1-Antibody ab14823. Therefore I am looking for the corresponding peptide to use.

    Read More
    Answer


    Unfortunately, we can not offer a blocking peptide for anti-NCR1 antibody [n1D9] (ab14823), since a recombinant full length protein corresponding to amino acids 22-255 of Human NCR1 was used as immunogen.


    I took a look through our catalog and would recommend human NCR1 full length protein (ab132141) or human NCR1 protein fragment (ab191681) for you as it fits your needs. The human NCR1 full length protein (ab132141) compromises the amino acids 22 to 303 and the human NCR1 protein fragment (ab191681) compromises amino acids from 22 to 254. Therefore we would expect either of these products to work with anti-NCR1 antibody [n1D9] (ab14823) as blocking peptide as well as positive control.



    You can see the datasheet and extra information here:

    https://www.abcam.com/Recombinant-Human-NCR1-protein-ab132141.html (or use the following: https://www.abcam.com/Recombinant-Human-NCR1-protein-ab132141.html).

    https://www.abcam.com/Recombinant-Human-NCR1-protein-ab191681.html (or use the following: https://www.abcam.com/Recombinant-Human-NCR1-protein-ab191681.html).

    Read More

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