Anti-NCOR2/SMRT 抗体 [1212] (ab2780)

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Key features and details

  • Mouse monoclonal [1212] to NCOR2/SMRT
  • Suitable for: IHC-Fr, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-NCOR2/SMRT antibody [1212]
    NCOR2/SMRT 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1212] to NCOR2/SMRT

  • 由来種

    Mouse

  • 特異性

    We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

  • アプリケーション

    適用あり: IHC-Fr, WB, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide corresponding to Human NCOR2/SMRT.
    Sequence:

    CGGA-SASDPHREKTQ


    (Peptide available as ab4909)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    Previously labelled as NCOR2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50.
WB 1/250. Detects a band of approximately 220-250 kDa (predicted molecular weight: 274 kDa).
ICC/IF Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能

    Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.

  • 組織特異性

    Ubiquitous. High levels of expression are detected in lung, spleen and brain.

  • 配列類似性

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.

  • ドメイン

    The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
    The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CTG 26 antibody
      • CTG repeat protein 26 antibody
      • CTG26 antibody
      • N CoR2 antibody
      • N-CoR2 antibody
      • NCoR 2 antibody
      • Ncor2 antibody
      • NCOR2_HUMAN antibody
      • Nuclear Receptor Co-Repressor 2 antibody
      • Nuclear receptor corepressor 2 antibody
      • retinoic-acid-receptor-associated corepressor antibody
      • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
      • Silencing mediator of retinoic acid and thyroid hormone receptor antibody
      • SMAP 270 antibody
      • SMAP270 antibody
      • SMRT antibody
      • SMRTE antibody
      • SMRTE tau antibody
      • T3 receptor associating factor antibody
      • T3 receptor-associating factor antibody
      • Thyroid retinoic acid receptor associated corepressor antibody
      • Thyroid- antibody
      • TNRC 14 antibody
      • TNRC14 antibody
      • TRAC 1 antibody
      • TRAC antibody
      • TRAC1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody [1212] (ab2780)
      Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody [1212] (ab2780)

      Immunolocalization of NCOR2/SMRT in HeLa cells using ab2780. 

    参考文献 (2)

    ab2780 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2780 は 2 報の論文で使用されています。

    • Yu J  et al. The corepressor silencing mediator for retinoid and thyroid hormone receptor facilitates cellular recovery from DNA double-strand breaks. Cancer Res 66:9316-22 (2006). WB . PubMed: 16982777
    • Mani S  et al. Activation of the steroid and xenobiotic receptor (human pregnane X receptor) by nontaxane microtubule-stabilizing agents. Clin Cancer Res 11:6359-69 (2005). PubMed: 16144941

    レビューと Q&A

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    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM High background, low signal and non specific staining SAMPLE HeLa cells PRIMARY ANTIBODY NoCR ab2780, diluted 1/100 in PBS 10% FBS ANTIBODY STORAGE CONDITIONS -20? FIXATION OF SAMPLE Paraformaldehyde 4%, 10 minutes room temperature PERMEABILIZATION STEP PBS-0.1 % Triton X-100 BLOCKING CONDITIONS PBS 10% FBS SECONDARY ANTIBODY Cy5 anti-mouse, diluted 1/100 in PBS 10% FBS

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    Answer

    I'm sorry to hear you are having a problem with ab2780. We have not had any complaints regarding this antibody, so I believe that I can give you some protocol tips to get the antibody working. I believe that the problem may be in the blocking step using 10% FBS, or a different fixation may also help. This antibody has been tested in immunohistochemistry (Frozen sections) in HeLa cells and I am assuming this is the application that you are using. I would like to suggest the following modifications to your protocol: 1) block using 10% normal goat serum 1% BSA in TBS for 2 hrs at room temp, 2) incubate in primary antibody at 1/50 in 1% NGS 1% BSA O/N at 4C, 3) incubate in secondary antibody in 1% NGS 1% BSA for 1 hr at room temp. Additionally, you could try fixing using ice cold acetone or methanol for 10 minutes instead of PFA, if the above suggestions do not work. Please let me know if this helps and do not hesitate to contact us for further advice,

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    Question

    Does this antibody recognize NCoR1 as well as NCoR2?

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    Answer

    This antibody has not been tested for cross-reactivity with NCoR antigens. We have an NCoR antibody, ab3482. If you have any further questions, please contact us again.

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