Anti-NCKAP1L 抗体 (ab272631)
Key features and details
- Rabbit polyclonal to NCKAP1L
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NCKAP1L antibody
NCKAP1L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NCKAP1L -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human NCKAP1L aa 1-100.
Database link: P55160 -
ポジティブ･コントロール
- WB: RT4, U-251MG sp, Human plasma (IgG/HSA depleted), Human liver tissue and tonsil tissue. IHC-P: Human lymph tissue, small intestine tissue, liver tissue, lung tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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組織特異性
Expressed only in cells of hematopoietic origin. -
配列類似性
Belongs to the HEM-1/HEM-2 family. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3071 Human
- Omim: 141180 Human
- SwissProt: P55160 Human
- Unigene: 182014 Human
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別名
- 4930568P13Rik antibody
- AI463083 antibody
- HEM1 antibody
see all
画像
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Western blot - Anti-NCKAP1L antibody (ab272631)Lanes 2-6 : Anti-NCKAP1L antibody (ab272631) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251MG sp
Lane 4 : uman plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue
Lane 6 : Human tonsil tissue -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human lymph tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human small intestine tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human liver tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1L antibody (ab272631)Paraffin-embedded formalin-fixed Human lung tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272631 は論文での使用が確認できていません。
