Anti-NCF1/p47-phox 抗体 (ab795)

Reviews (2)Q&A (4)References (10)

Key features and details

  • Goat polyclonal to NCF1/p47-phox
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-NCF1/p47-phox antibody
    NCF1/p47-phox 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to NCF1/p47-phox

  • 由来種

    Goat

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Cow, Pig

  • 免疫原

    Synthetic peptide corresponding to Human NCF1/p47-phox aa 378-390 (C terminal).
    Sequence:

    CSESTKRKLASAV


    (Peptide available as ab22781)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • U937 cell lysates

  • 特記事項

    Principal Names - NCF1 / p47-phox; NCF1; p47phox; Neutrophil cytosolic factor-1 (47 kDa, chronic granulomatous disease, autosomal 1) Official Gene Symbol - NCF1 GenBank Accession Number – NP_000256 LocusLink ID - 4687

     

    NCF1 / p47-phox is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.

    Previously labelled as NCF1. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

1 hour primary incubation is recommended for this product.

ターゲット情報

  • 機能

    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

  • 関連疾患

    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

  • 配列類似性

    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

画像

  • Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Anti-NCF1/p47-phox antibody (ab795) at 1/0.5 dilution + U937 lysate in RIPA buffer. at 35 µg

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  • Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Western blot - Anti-NCF1/p47-phox antibody (ab795)

参考文献

ab795 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab795 は 10 報の論文で使用されています。

  • Wu W  et al. Xian-Ling-Gu-Bao induced inflammatory stress rat liver injury: Inflammatory and oxidative stress playing important roles. J Ethnopharmacol 239:111910 (2019). PubMed: 31026554
  • Srivastava S  et al. Mycobacterium tuberculosis PPE2 Protein Interacts with p67phox and Inhibits Reactive Oxygen Species Production. J Immunol 203:1218-1229 (2019). PubMed: 31375544
  • Ren Y  et al. Si-Miao-Yong-An decoction ameliorates cardiac function through restoring the equilibrium of SOD and NOX2 in heart failure mice. Pharmacol Res 146:104318 (2019). PubMed: 31228552
  • Kong M  et al. Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription. Redox Biol 26:101302 (2019). PubMed: 31442911
  • Yang HY  et al. LOX-1 is implicated in oxidized low-density lipoprotein-induced oxidative stress of macrophages in atherosclerosis. Mol Med Rep 12:5335-41 (2015). PubMed: 26165515
  • Lu XY  et al. Acute ethanol induces apoptosis by stimulating TRPC6 via elevation of superoxide in oxygenated podocytes. Biochim Biophys Acta 1853:965-74 (2015). PubMed: 25601712
  • Jha P  et al. Genome-wide methylation profiling identifies an essential role of reactive oxygen species in pediatric glioblastoma multiforme and validates a methylome specific for H3 histone family 3A with absence of G-CIMP/isocitrate dehydrogenase 1 mutation. Neuro Oncol N/A:N/A (2014). Human . PubMed: 24997139
  • Song X  et al. Lovastatin inhibits human B lymphoma cell proliferation by reducing intracellular ROS and TRPC6 expression. Biochim Biophys Acta 1843:894-901 (2014). PubMed: 24518247
  • Giefing M  et al. Hodgkin-Reed-Sternberg cells in classical Hodgkin lymphoma show alterations of genes encoding the NADPH oxidase complex and impaired reactive oxygen species synthesis capacity. PLoS One 8:e84928 (2013). WB . PubMed: 24376854
  • Underhill DM  et al. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 106:2543-50 (2005). PubMed: 15956283

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Western blot abreview for Anti-NCF1/p47-phox antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 01 2016

Western blot abreview for Anti-NCF1/p47-phox antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 01 2016

Question

Are there any antibodies available that detect the phosphorylated form of p47phox (a regulatory NADPH oxidase subunit) in mouse tissue? I have attached a recent paper which has published in antioxidants and redox signaling. Authors used an antibody from your company for the detection of phospho p47phox in cells extracted from humans.



Thanks,

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Answer

Thank you for contacting us.

Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab111855, ab115564, ab795, ab63554, ab107125. Please check the datasheets and let me know, if you want me to create a performa invoice for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestandteile (p47PHOX, p40PHOX, p67PHOX, P22PHOX, gp91PHOX, Rac1/2, Rho-GDI, …), sowie (falls möglich) die Einzelkomponenten selber (für die Arbeit im zellfreien System). Können Sie uns weiterhelfen? Über ein Angebot würden wir uns freuen.

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Answer

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/index.html?datasheet=109366). Click here (or use the following: https://www.abcam.com/index.html?datasheet=80508). Click here (or use the following: https://www.abcam.com/index.html?datasheet=33186). Click here (or use the following: https://www.abcam.com/index.html?datasheet=2244). Click here (or use the following: https://www.abcam.com/index.html?datasheet=118159). Click here (or use the following: https://www.abcam.com/index.html?datasheet=75941). Click here (or use the following: https://www.abcam.com/index.html?datasheet=115855). Click here (or use the following: https://www.abcam.com/index.html?datasheet=59355). Click here (or use the following: https://www.abcam.com/index.html?datasheet=111855). Click here (or use the following: https://www.abcam.com/index.html?datasheet=795). Click here (or use the following: https://www.abcam.com/index.html?datasheet=88031). Click here (or use the following: https://www.abcam.com/index.html?datasheet=98054). Click here (or use the following: https://www.abcam.com/index.html?datasheet=117065). Click here (or use the following: https://www.abcam.com/index.html?datasheet=114183). Click here (or use the following: https://www.abcam.com/index.html?datasheet=84657). Click here (or use the following: https://www.abcam.com/index.html?datasheet=40471). Click here (or use the following: https://www.abcam.com/index.html?datasheet=89246). Click here (or use the following: https://www.abcam.com/index.html?datasheet=82670). Click here (or use the following: https://www.abcam.com/index.html?datasheet=51014). Click here (or use the following: https://www.abcam.com/index.html?datasheet=84655). Click here (or use the following: https://www.abcam.com/index.html?datasheet=78684). Click here (or use the following: https://www.abcam.com/index.html?datasheet=87665). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/index.html?datasheet=65349). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question

I would like to test this antibody in rat.

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Answer

Thank you very much for your interest in ab795. To our knowledge, ab795 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab795 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab795 in rat. I will then send a discount code. This code must be issued before purchasing ab795 so please wait for my reply before ordering. 2. Purchase ab795 either by phone, fax, or online (www.abcam.com). 3. Test it in rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab795 turns out to be unsuitable for rat you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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Question

I am interested in purchasing your NCF1 antibody (ab795). Do you have any information regarding its suitability for immunoprecipitation techniques.

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Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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