Anti-NCAM1 抗体 [RNL-1] (ab9018)

The antibodies mentioned above are suitable for Rat as well as human samples. ab9018 is yet to be tested in IHC-Fr however as it is tested in IHC-FoFr so it is more likely to work.

The suitable secondary's are for ab4648, ab82259 and ab9018 are

https://www.abcam.com/rabbit-polyclonal-secondary-antibody-to-mouse-igg-hl-hrp-ab97046.html

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg-hl-hrp-ab97023.html

for ab18258 it is

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-ab6721.html

for ab5392 it is;

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-chicken-igy-h-l-hrp-ab97135.html

The protocols are:

https://www.abcam.com/index.html?pageconfig=resource&rid=11417

https://www.abcam.com/index.html?pageconfig=resource&rid=11383

https://www.abcam.com/index.html?pageconfig=resource&rid=13046

IHC-Fr is immunohistochemistry on frozen section

IHC-FoFr is PFA perfusion fixed frozen sections.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for a refund for you.

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for taking the time to contact me again and provide an update. I am sincerely sorry to hear you have also had difficulty obtaining satisfactory results from the replacement vial of antibody.

I appreciate your concerns, this is disappointing. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results, so I am not able to suggest anything further. I can reassure you that your enquiries will be kept on our quality monitoring records for our ongoing reference.

I apologize for the inconvenience. In this case, I suggest it would be appropriate to provide a refund or a credit note for purchase of another product.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed this time.

Thank you for your message.

I fully understand your concerns and would like to provide some information that I hope will give some reassurance. I can confirmthat we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

Therefore, I hope the replacement vial should work well. However, please note that the replacement will be covered by the 6 month guarantee should you encounter any problems. I would encourage you to contact me as soon as possible in this case.

I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1143353.

To check the status of the order please contact our Customer Service team and reference this number.

With regards to suggests positive controls, I can confirm that rat brain tissue should be a suitable positive control for this protein. There are some images from IHC staining of rat brain on the datasheet, also mouse brain. HT1080 cells woudl also be suitable.

According to the Unigene EST expression profile, NCAM is expressed in brain, adrenal gland, ear, larynx and lung, and to a lower extent in many other organs:


http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.503878

Please note that the free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement or credit note in compensation.

In addition, I can suggest you may like to consider the following suggestions as a check for future experiments:

1. Antigen retrieval can sometimes require some optimization. I can recommend to try citrate buffer for 2, 5, 10 and 20 minutes, or enzymatic retrieval.

2. I can recommend to try BSA or serum rather than Caesin to block. Changing blocking agent can sometimes help to improve the results.

3. Are the current vials of secondary antibody working well with other primary antibodies?

4. I can suggest to consider Including a permeabilization step which would help to improve the results.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forIHC-P andIHC-FoFr and in mouse and rat samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if youare also able to provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

No staining was seen on any slides

Sample (Species/Tissue/Cell Type/Cell Line etc.)

mouse skeletal muscle tissue

Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)Paraffin embedded or frozen sections?

Antigen retrieval (Enzymatic method, Heat mediated technique etc.)

Tris-EDTA pH8 buffer


Has citrate buffer retrieval been tried? What different time points were tried for optimization?

Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)

blocking in 1xCaesin, same primary antibodies titrations and detection

I can recommend to try BSA or serum rather than Caesin to block. Changing blocking agent can sometimes help to improve the results.

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

1:50, 1:100 and 1:200 in Dako Diluent (S0809) overnight at 4C

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)

Rat brain tissue that was frozen and once again, no signal was seen

Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No


Is the current vial of secondary antibody working well with other primary antibodies?


Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide an image which would help us to assess the results

Thank you for contacting us.

I suggest having a look at some of the references for our NCAM antibodies such as ab9018, to see if this marker will be appropriate for what you are trying to do. It is not clear to me if NCAM is on all neurons or just a subset, though.

Click here (or use the following: https://www.abcam.com/NCAM1-antibody-RNL-1-ab9018.html).

All other markers that I know of are intracellular.

While searching for marker candidates, I came across a paper which appears to have a good discussion of tissue dissociation techniques,

Stem Cells. 2007 Jun;25(6):1560-70.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. The above amount will be credited back to your credit card. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for sending those images. Comparing the images you sent and the images from the Allen Institute for Brain Science, see link below: http://mouse.brain-map.org/gene/show/17734 It would appear that your staining is similar to that theirs, maybe a little stronger but it all seems to be good (as long as I am reading you slides correctly and you were looking at the Cerebellum). To try and reduce the intensity of the staining, you could lower the primary antibody concentration to 1/150 and also block for longer. Please let me know if there is anything else I can help you with.