Anti-NCAM1 抗体 [CAL53] (ab237708)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL53] to NCAM1
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NCAM1 antibody [CAL53]
NCAM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [CAL53] to NCAM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IP, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human brain and cerebellum lysates; mouse cerebellum lysate; rat brain lysate; Neuro-2a whole cell lysate. IHC-P: Human cerebrum, glioma, pancreas and pancreatic carcinoma tissue; mouse cerebrum tissue; rat cerebrum tissue. ICC/IF: Neuro-2a cells. IP: SH-SY5Y whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.50
Preservative: 0.05% Sodium azide
Constituent: 99.95% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purity >99% -
ポリ/モノ
モノクローナル -
クローン名
CAL53 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237708の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 140 kDa).
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ICC/IF |
1/50.
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IP |
1/20.
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IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 140 kDa). |
ICC/IF
1/50. |
IP
1/20. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
配列類似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4684 Human
- Entrez Gene: 17967 Mouse
- Entrez Gene: 24586 Rat
- Omim: 116930 Human
- SwissProt: P13591 Human
- SwissProt: P13595 Mouse
- SwissProt: P13596 Rat
- Unigene: 503878 Human
see all -
別名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
画像
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All lanes : Anti-NCAM1 antibody [CAL53] (ab237708) at 1/1000 dilution
Lane 1 : Human brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : Neuro-2a (mouse neuroblastoma cell line0 whole cell lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 140 kDa
Observed band size: 140-180 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NCAM1 with ab237708 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the human cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling NCAM1 with ab237708 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing membranous staining in Neuro-2a cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
PCS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Negative control: L-929 (PMID:9696812).
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NCAM1 with ab237708 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on islet in the mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NCAM1 with ab237708 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on islet in the rat cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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NCAM1 was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab237708 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab237708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: SH-SY5Y whole cell lysate 10 μg (Input).
Lane 2: ab237708 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237708 in SH-SY5Y whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab237708 は 1 報の論文で使用されています。
- Shiwaku H et al. Autoantibodies against NCAM1 from patients with schizophrenia cause schizophrenia-related behavior and changes in synapses in mice. Cell Rep Med 3:100597 (2022). PubMed: 35492247