NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
製品の概要
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製品名
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen
Alkaline Phosphatase Chromogen ihc 試薬 製品一覧 -
アプリケーション
適用あり: WB, Dot blotmore details -
特記事項
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.
This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
Constituents: 0.09% NBT, 0.09% 5-bromo-4-chloro-3-indolyl-phosphate-p-toluidine salt -
Concentration information loading...
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研究分野
関連製品
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Unmasking reagent
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7468の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1.
Ready to use formulation. BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
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Dot blot |
1/1.
Ready to use formulation. |
特記事項 |
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WB
1/1. Ready to use formulation. BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
Dot blot
1/1. Ready to use formulation. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab7468 は 3 報の論文で使用されています。
- Gigout A et al. Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species. Arthritis Res Ther 23:113 (2021). PubMed: 33853645
- Hassani A et al. Epstein-Barr virus is present in the brain of most cases of multiple sclerosis and may engage more than just B cells. PLoS One 13:e0192109 (2018). PubMed: 29394264
- Song JW et al. 3'-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli. Sci Rep 6:29406 (2016). WB . PubMed: 27406241