Anti-Mesothelin 抗体 [SP74] (ab93620)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP74] to Mesothelin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Mesothelin antibody [SP74]
Mesothelin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP74] to Mesothelin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig -
免疫原
Synthetic peptide within Human Mesothelin aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13421 -
ポジティブ・コントロール
- IHC-P: Human Mesothelioma tissue. WB: BxPC-3, HeLa, PC3 and A549 cell lysate.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP74 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93620の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.322 µg/ml. Predicted molecular weight: 69 kDa.
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IHC-P | (2) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.322 µg/ml. Predicted molecular weight: 69 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
組織特異性
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
関連疾患
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
配列類似性
Belongs to the mesothelin family. -
翻訳後修飾
Both MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
細胞内局在
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 10232 Human
- Entrez Gene: 100524016 Pig
- Omim: 601051 Human
- SwissProt: Q13421 Human
- Unigene: 408488 Human
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別名
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
see all
画像
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All lanes : Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate
Lane 3 : PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 69 kDa
Observed band size: 49,69 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab93620 は 3 報の論文で使用されています。
- Kim H et al. Mesothelin expression and its prognostic role according to microsatellite instability status in colorectal adenocarcinoma. Medicine (Baltimore) 98:e16207 (2019). PubMed: 31261569
- Smith TT et al. Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors. J Clin Invest 127:2176-2191 (2017). PubMed: 28436934
- Sopha SC et al. Diagnostic and therapeutic implications of a novel immunohistochemical panel detecting duodenal mucosal invasion by pancreatic ductal adenocarcinoma. Int J Clin Exp Pathol 6:2476-86 (2013). PubMed: 24228110