製品名Natural Myelin Basic Protein (Biotin)
Myelin Basic Protein タンパク質・ペプチド 製品一覧
タンパク質長Full length protein
配列aaqkrpsqrs kylasastmd harhgflprh rdtgildslg rffgsdrgap krgsgkdghh aartthygsl pqkaqghrpq denpvvhffk nivtprtppp sqgkgrglsl srfswgaegq kpgfgyggra sdyksahkgl kghdaqgtls kifklggrds rsgspmarr
領域1 to 169
Our Abpromise guarantee covers the use of ab792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Acting as substrate of MAP Kinase such as ERK1 and ERK2
備考Myelin basic protein (MBP) is isolated from bovine brain. It is well documented that MBP is a substrate for many protein kinases including PKC, PKA, and MAPK family (such as ERK1/2). Biotinylated MBP is an excellent substrate for the solid-phase kinase assay system, facilitating the separation of free ATP and the kinases. Biotinylated MBP could enhance the efficiency of immobilization of the substrate on an avidin-coated microplate and the substrate will be readily accessible for the kinase activity through a large molecule avidin as a spacer. We do not sell non-biotinylated MBP.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 2.09% MOPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Golli MBP
- Golli MBP; myelin basic protein
機能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
組織特異性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
関連疾患Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
配列類似性Belongs to the myelin basic protein family.
発生段階Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
翻訳後修飾Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
細胞内局在Myelin membrane. Cytoplasmic side of myelin.
- Information by UniProt
MBP and Biotinylated MBP was phosphorylated with 0.05ug of active ERK1 with P32 radiolabeled ATP.
Assay reference: Sanghera JS et al., J Immunol. (1996) 156:4457-4465
Activity of biotinylated MBP
Lane a (5 ug of biotinylated MBP): 33682 counts/min
Lane b (2.5 ug of biotinylated MBP): 18478 counts/min
Lane c (1.25 ug of biotinylated MBP): 6714 counts/min
Lane d (0.625 ug of biotinylated MBP): 2889 counts/min
Lane e (0.36 ug of biotinylated MBP): 1039 counts/min
Lane f (0.18 ug of biotinylated MBP): 633 counts/min
Control Lane (0.0 ug of biotinylated MBP): 145 counts/min
Non-biotinylated MBP Activity
Lane A (5 ug of biotinylated MBP): 37604 counts/min
Lane B (2.5 ug of biotinylated MBP): 17824 counts/min
Lane C (1.25 ug of biotinylated MBP): 8867 counts/min
Lane D (0.625 ug of biotinylated MBP): 4190 counts/min
Lane E (0.36 ug of biotinylated MBP): 2248 counts/min
Lane F (0.18