Native Human Fibronectin protein (ab80021)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Native Human Fibronectin protein
Fibronectin タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purified from human plasma -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
配列の追加情報
Molecular weight: the MW of the hetrodimer is 550 kDa
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab80021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.476% HEPES, 0.87% Sodium chloride -
再構成Reconstitute with 1.0 milliliters of water to achieve 2.0 mg/mL. Human Fibronectin is known for its solubility issues. The best way to get this material into solution, after reconstitution, is to incubate in a 37ºC water bath for at least 30 minutes. Typically this is sufficient, however a small amount of un-dissolved material may remain. If this is the case, a few gentle aspirations with a pipette is recommended.
関連情報
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別名
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
機能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
組織特異性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
関連疾患
Glomerulopathy with fibronectin deposits 2 -
配列類似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
発生段階
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻訳後修飾
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
画像
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Gel: Novex 4-12% Bis-Tris
Load Load: Human Fibronectin, 1 µg per lane in Non-reducing (NR) and reducing (R) conditions.
Buffer: MOPS
MW Ladder: Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab80021 は論文での使用が確認できていません。