Anti-Natriuretic peptides A 抗体 [M622709] (ab2093)

Reviews (2)Q&A (5)References (1)

製品の概要

  • 製品名

    Anti-Natriuretic peptides A antibody [M622709]
    Natriuretic peptides A 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [M622709] to Natriuretic peptides A

  • 由来種

    Mouse

  • 特異性

    Affinity Constant: 2.2 x 1010 L/M

  • アプリケーション

    適用あり: Flow Cyt, RIA, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Atrial Natriuretic Peptide whole molecule

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 20080656

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RIA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • 関連疾患

    Atrial standstill 2
    Atrial fibrillation, familial, 6

  • 配列類似性

    Belongs to the natriuretic peptide family.

  • 翻訳後修飾

    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ANF antibody
      • ANF_HUMAN antibody
      • ANP antibody
      • ATFB6 antibody
      • Atrial natriuretic factor antibody
      • Atrial natriuretic peptide antibody
      • Atriopeptin antibody
      • Cardiodilatin antibody
      • Cardiodilatin related peptide antibody
      • Cardiodilatin-related peptide antibody
      • Cardionatrin antibody
      • CDD ANF antibody
      • CDD antibody
      • CDD-ANF antibody
      • CDP antibody
      • Natriuretic peptide A antibody
      • Natriuretic peptide precursor A antibody
      • NPPA antibody
      • PND antibody
      • Prepronatriodilatin antibody
      see all

    参考文献

    This product has been referenced in:

    • Therrien JP  et al. A gene therapy approach for long-term normalization of blood pressure in hypertensive mice by ANP-secreting human skin grafts. Proc Natl Acad Sci U S A 107:1178-83 (2010). IHC-P, Flow Cyt ; Human . Read more (PubMed: 20080656) »
    See 1 Publication for this product

    ab2093 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic peptides A antibody [M622709]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Salivary gland)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Salivary gland
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Isabelle Miletich

    Verified customer

    投稿 Jan 05 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic peptides A antibody [M622709]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart tissue including atrium)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Heart tissue including atrium
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21D°C
    Fixative
    Formaldehyde
    Read More

    Dr. Isabelle Miletich

    Verified customer

    投稿 Nov 04 2016

    Question

    Customer has requested MSDS and CoA for their order #1156531. There are 2 items on the order, and they are as follows: Anti-ANP antibody [M622709] (ab2093) Anti-Urodilatin antibody (ab14319) Customer info is on the order. Thanks :)

    Read More
    Answer

    Thank you for contacting us.


    I have attached the CoCs for ab14319, Anti-Urodilatin antibody and for ab2093 Anti-ANP antibody [M622709] as well the SDS for ab2093. I have also asked our documents team to create the SDS for ab14319. This will be added to the webpage for this product very early next week and can be downloaded directly from there. The page for this product can be found at this link:

    https://www.abcam.com/urodilatin-antibody-ab14319.html


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Use our products? Submit an Abreview. Earn rewards!

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    Question

    I would like to know if the purified form of the whole ANP protein that has been used to raise this antibody is available commercially. Thanks,

    Read More
    Answer

    Thank you for your enquiry. Unfortunately, we do not have the protein that was used to make this antibody in our catalogue and I am afraid that I do not know if it is commercially available. I am sorry I can't be of more assistance in this occasion. Should you require anything further, please do not hesitate to contact me.

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    Question

    What's the antigen sequence for this antibody? What part of the protein was the antibody made to?

    Read More
    Answer

    Thank you for your enquiry. ab2093 was generated using Atrial Natriuretic Peptide whole molecule as the immunogen. The peptide sequence has accession number: CAA25700 and the following sequence: MSSFSTTTVS FLLLLAFQLL GQTRANPMYN AVSNADLMDF KNLLDHLEEK MPLEDEVVPP QVLSEPNEEA GAALSPLPEV PPWTGEVSPA QRDGGALGRG PWDSSDRSAL LKSKLRALLT APRSLRRSSC FGGRMDRIGA QSGLGCNSFR YRR I hope this information helps, please do not hesitate to contact me should you require further assistance.

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    Question

    Customer would like to know - what is the molecular weight of this antibody?

    Read More
    Answer

    Clone M622709 is subtype IgG1. The approximate molecular weight of IgG1 is 148,000 daltons. This antibody has only been tested for application in Immunohistochemistry (Formalin-fixed paraffin-embedded sections)and Radioimmunoassay.

    Read More

    Question

    I am using this antibody in WB on human left ventricle whole cell extract. I am getting a band at 40kDa, where the expected molecular weight should be approximately 17kDa. I'm wondering if it is detecting a precursor form of ANP? Any help you could give me would be very much appreciated.

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. As my colleagues possibly explained to you over the telephone, this antibody has not been tested for Western blot application. According to the datasheet, ab2093 recognizes the native form of the human protein (it is suitable for IHC and RIA) but so far we do not have any data regarding its suitability for WB. It may well be that the antibody recognizes the precursor form of ANP. It is also possible that the protein is undergone some post-translational modification, however we do not have any information about it. If you need anything further or any help then please let us know.

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