Native Staphylococcus aureus Protein A (FITC) (ab7455)

Submit a review Q&A (3)References (1)

Key features and details

  • Expression system: Native
  • Purity: > 95% Protein A purified
  • Suitable for: Flow Cyt, Immunomicroscopy

製品の詳細

  • 製品名

    Native Staphylococcus aureus Protein A (FITC)
    Protein A タンパク質・ペプチド 製品一覧

  • 精製度

    > 95 % Protein A purified.

  • 発現系

    Native

  • アクセッション番号

    P38507

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Native

    • 生物種

      Staphylococcus aureus

    • 予測される分子量

      56 kDa

  • 標識

    FITC. Ex: 493nm, Em: 528nm

特性

Our Abpromise guarantee covers the use of ab7455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Flow Cytometry

    Immunomicroscopy

  • 製品の状態

    Liquid

  • 備考

    Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, BSA

関連情報

  • 別名

    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A

  • 関連性

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • 細胞内局在

    Secreted; cell wall.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7455 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7455 は 1 報の論文で使用されています。

  • He B  et al. Group A Rotaviruses in Chinese Bats: Genetic Composition, Serology, and Evidence for Bat-to-Human Transmission and Reassortment. J Virol 91:N/A (2017). IF . PubMed: 28381569

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Protein G-FITC conjugated (ab7455)
Protein A- FITCconjugated (ab7459)
Do you have APC and PE conjugated Protein G and A products?
Do you have recombinant anti human IgG1?

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Answer

Thank you very much for having patience.

My colleague has tried to source these products form our collaborator supplier however due to non availability of these product we are unable to add these in catalogue.

You can however use the following kits to conjugate the protein yourselves. Please check the following products

ab102851, ab102849, ab102850, ab102848

ab102912, ab102870

Please click the below given link for full list;

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

We unfortunately do not have recombinant anti human IgG1 antibody available.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Question

Protein G-FITC conjugated (ab7455)
Protein A- FITCconjugated (ab7459)
Do you have APC and PE conjugated Protein G and A products?
Do you have recombinant anti human IgG1?

Read More
Answer

Thank you for contacting us.

I can confirm that I have forwarded your inquiry to my colleague, who is protein product manager at Abcam. She is now looking into the requested product line and will be contacting me with updates soon.

I will get back to you, as soon as I get a reply from her.

Thank you very much for having patience!

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Question

Can you please provide a certificate of origin for this product, or other regulatory information about possible contamination of this product with Mad Cow Disease?

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Answer

Thank you for contacting us. To my knowledge we do not have a prepared certificate of origin document for this protein. However, this product was derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. I hope this helps, please let me know if you need any other information.

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