Native Protein A (Alkaline Phosphatase) (ab7345)
Key features and details
- Expression system: Native
- Purity: > 95% Protein A purified
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr
製品の詳細
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製品名
Native Protein A (Alkaline Phosphatase)
Protein A タンパク質・ペプチド 製品一覧 -
精製度
> 95 % Protein A purified. -
発現系
Native -
タンパク質長
Full length protein -
Animal free
No -
由来
Native -
標識
Alkaline Phosphatase
特性
Our Abpromise guarantee covers the use of ab7345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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製品の状態
Liquid -
備考
Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1.5% BSA
BSA is IgG and Protease free
関連情報
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別名
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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関連性
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
細胞内局在
Secreted; cell wall.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab7345 は論文での使用が確認できていません。